CDK2-IN-23 (Compound 17) is a kinase-selective and highly potent CDK2 inhibitor (IC50 = 0.29 nM). CDK2-IN-23 exhibits pharmacodynamic inhibition of CDK2 in a CCNE1 amplification mouse model. CDK2-IN-23 can be used in cancer research.

Physicochemical Properties

Molecular Formula	C20H27F3N6O3S
Molecular Weight	488.53
CAS #	2488073-20-5
Appearance	Solid powder ; White to off-white
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Discovery of (4-Pyrazolyl)-2-aminopyrimidines as Potent and Selective Inhibitors of Cyclin-Dependent Kinase 2. J Med Chem. 2024 Feb 22;67(4):3112-3126.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~204.70 mM; with ultrasonication)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 5 mg/mL (10.23 mM) (saturation unknown) in 10% DMSO 40% PEG300 5% Tween-80 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution and add it to 400 μL PEG300 and mix well. Then add 50 μL Tween-80 to the above system and mix well. Then continue to add 450 μL of physiological saline to make up to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 5 mg/mL (10.23 mM)(saturation unknown) in 10% DMSO 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared,you can add 100 μL of 50.0 mg/mL clear DMSO stock solution and add it to 900 μL of 20% SBE-β-CD saline solution and mix well.。 Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0470 mL	10.2348 mL	20.4696 mL
	5 mM	0.4094 mL	2.0470 mL	4.0939 mL
	10 mM	0.2047 mL	1.0235 mL	2.0470 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.