CBR-470-2 is a glycine-substituted analog that activates/agonizes NRF2 signaling. CBR-470-2 may be utilized to regulate glycolysis.

Physicochemical Properties

Molecular Formula	C12H13CL2NO6S2
Molecular Weight	402.27071928978
Exact Mass	400.956
CAS #	2416095-00-4
PubChem CID	155819525
Appearance	White to off-white solid powder
LogP	-0.9
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	5
Heavy Atom Count	23
Complexity	654
Defined Atom Stereocenter Count	2
SMILES	ClC1=C(C=CC(=C1)S([C@H]1CS(C[C@@H]1NCC(=O)O)(=O)=O)(=O)=O)Cl
InChi Key	FNQDSYKGBCVHHI-QWRGUYRKSA-N
InChi Code	InChI=1S/C12H13Cl2NO6S2/c13-8-2-1-7(3-9(8)14)23(20,21)11-6-22(18,19)5-10(11)15-4-12(16)17/h1-3,10-11,15H,4-6H2,(H,16,17)/t10-,11-/m0/s1
Chemical Name	2-[[(3S,4R)-4-(3,4-dichlorophenyl)sulfonyl-1,1-dioxothiolan-3-yl]amino]acetic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	NRF2[1]
ln Vitro	In epidermal keratinocytes and dermal fibroblasts, CBR-470-2 (1-10 μM; 24 h) raises transcript levels of the NRF2-responsive genes NQO1 and HMOX1[1].
ln Vivo	In vivo, CBR-470-2 (50 mg/kg; po twice daily for 10 d) causes NRF2 signaling to become activated[1].
Animal Protocol	Animal/Disease Models: balb/c (Bagg ALBino) mouse (5-week old) are exposed to UVB[1] Dosage : 50 mg/kg Route of Administration: Po twice (two times) daily for 10 days Experimental Results: Resulted in comparable beneficial effects on erythema histological scores and total wounded area. diminished epidermal thickness in response to UV exposure.
References	[1]. A metabolite-derived protein modification integrates glycolysis with KEAP1-NRF2 signalling. Nature. 2018 Oct;562(7728):600-604.

Solubility Data

Solubility (In Vitro)

DMSO :~200 mg/mL (~497.18 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 5 mg/mL (12.43 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.4859 mL	12.4295 mL	24.8589 mL
	5 mM	0.4972 mL	2.4859 mL	4.9718 mL
	10 mM	0.2486 mL	1.2429 mL	2.4859 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.