CB-7921220 is an inhibitor (blocker/antagonist) of adenylate cyclase.

Physicochemical Properties

Molecular Formula	C14H12N2O2
Molecular Weight	240.257283210754
CAS #	115453-99-1
PubChem CID	5740547
Appearance	Pink to red solid powder
Melting Point	225 - 226 °C
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	3
Heavy Atom Count	18
Complexity	309
Defined Atom Stereocenter Count	0
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Adenylyl cyclase (AC) 1 has some isoform selectivity in CB-7921220, yet it is unable to distinguish between AC1 and AC6. The lack of selectivity of CB-7921220 between AC1 and AC6 may be explained by its more consistently predicted binding position in the two virtual docking screens and similar binding conformation to ATP and P-site inhibitors [1].
References	[1]. Isoform selectivity of adenylyl cyclase inhibitors: characterization of known and novel compounds. J Pharmacol Exp Ther. 2013 Nov;347(2):265-75.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~208.11 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (10.41 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.1622 mL	20.8108 mL	41.6216 mL
	5 mM	0.8324 mL	4.1622 mL	8.3243 mL
	10 mM	0.4162 mL	2.0811 mL	4.1622 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.