Bromocresol green is a pH-sensitive triphenylmethane dye extensively usedto determine protein and albumin concentrations in serum. It is an alkaloid dye that appears yellow-green to blue-green as the pH increases from 3.0 to 7.5. Bromocresol green turns yellow (λmax=435 nm, protonated form) when placed in an acidic solution (e.g. pH=4.15) and appears blue (λmax=615 nm, deprotonated form) in an alkaline solution form). Therefore, Bromocresol green could be utilized as a pH indicator and is extensively used in the field of biochemical analysis. In addition, Bromocresol green is also used to detect the concentration of molecules such as creatinine and determine the survival rate of cells.

Physicochemical Properties

Molecular Formula	C21H14BR4O5S
Molecular Weight	698.01
Exact Mass	693.729
CAS #	76-60-8
PubChem CID	6451
Appearance	Off-white to light brown solid powder
Density	2.1±0.1 g/cm3
Boiling Point	626.0±55.0 °C at 760 mmHg
Melting Point	225 °C
Flash Point	332.4±31.5 °C
Vapour Pressure	0.0±1.9 mmHg at 25°C
Index of Refraction	1.716
LogP	7.8
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	2
Heavy Atom Count	31
Complexity	742
Defined Atom Stereocenter Count	0
SMILES	BrC1C(=C(C([H])=C(C=1C([H])([H])[H])C1(C2=C([H])C(=C(C(=C2C([H])([H])[H])Br)O[H])Br)C2=C([H])C([H])=C([H])C([H])=C2S(=O)(=O)O1)Br)O[H]
InChi Key	FRPHFZCDPYBUAU-UHFFFAOYSA-N
InChi Code	InChI=1S/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3
Chemical Name	2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxy-2-methylphenyl)-1,1-dioxo-2,1λ6-benzoxathiol-3-yl]-3-methylphenol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Delanghe S, et al. Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients. Clin Chem Lab Med. 2018 Feb 23;56(3):436-440. [2]. Jurmanović S, et al. Organically modified silicate thin films doped with colourimetric pH indicators methyl red and bromocresol green as pH responsive sol–gel hybrid materials[J]. Thin Solid Films, 2010, 518(8): 2234-2240. [3]. Chaiyo S, et al. A novel paper-based colorimetry device for the determination of the albumin to creatinine ratio. Analyst. 2018 Nov 5;143(22):5453-5460. [4]. Hou H, et al. Single-cell pH imaging and detection for pH profiling and label-free rapid identification of cancer-cells. Sci Rep. 2017 May 11;7(1):1759.

Additional Infomation

Bromocresolgreen is a member of benzofurans. An indicator and reagent. It has been used in serum albumin determinations and as a pH indicator. See also: BCG vaccine (USP) (annotation moved to).

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (143.26 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (3.58 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.4326 mL	7.1632 mL	14.3264 mL
	5 mM	0.2865 mL	1.4326 mL	2.8653 mL
	10 mM	0.1433 mL	0.7163 mL	1.4326 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.