Bradykinin (2-9) is an amino-truncated kinin peptide. Bradykinin (2-9) is a Bradykinin metabolite formed by aminopeptidase P cleavage.

Physicochemical Properties

Molecular Formula	C44H61N11O10
Molecular Weight	904.02300
Exact Mass	903.46
CAS #	16875-11-9
PubChem CID	14694060
Appearance	White to off-white solid powder
Density	1.47g/cm3
Index of Refraction	1.684
LogP	0.951
Hydrogen Bond Donor Count	10
Hydrogen Bond Acceptor Count	12
Rotatable Bond Count	22
Heavy Atom Count	65
Complexity	1700
Defined Atom Stereocenter Count	7
SMILES	C1C[C@H](NC1)C(=O)N2CCC[C@H]2C(=O)NCC(=O)N[C@@H](CC3=CC=CC=C3)C(=O)N[C@@H](CO)C(=O)N4CCC[C@H]4C(=O)N[C@@H](CC5=CC=CC=C5)C(=O)N[C@@H](CCCN=C(N)N)C(=O)O
InChi Key	ZZHVXIPXTCBVBE-POFDKVPJSA-N
InChi Code	InChI=1S/C44H61N11O10/c45-44(46)48-20-8-16-30(43(64)65)51-38(59)32(24-28-13-5-2-6-14-28)52-40(61)35-18-10-22-55(35)42(63)33(26-56)53-37(58)31(23-27-11-3-1-4-12-27)50-36(57)25-49-39(60)34-17-9-21-54(34)41(62)29-15-7-19-47-29/h1-6,11-14,29-35,47,56H,7-10,15-26H2,(H,49,60)(H,50,57)(H,51,59)(H,52,61)(H,53,58)(H,64,65)(H4,45,46,48)/t29-,30-,31-,32-,33-,34-,35-/m0/s1
Chemical Name	(2S)-5-(diaminomethylideneamino)-2-[[(2S)-2-[[(2S)-1-[(2S)-3-hydroxy-2-[[(2S)-3-phenyl-2-[[2-[[(2S)-1-[(2S)-pyrrolidine-2-carbonyl]pyrrolidine-2-carbonyl]amino]acetyl]amino]propanoyl]amino]propanoyl]pyrrolidine-2-carbonyl]amino]-3-phenylpropanoyl]amino]pentanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

Bradykinin is a tissue-based system that has strong effects on the heart and kidneys. A highly sensitive amino-terminal directed radioimmunoassay that detects bradykinin-(1-7), bradykinin-(1-8) using high-performance liquid chromatography, and bradykinin-(1-9) was created in order to examine the regulation of this system. Bradykinin was identified using carboxyl-terminal guided radioimmunoassay in rat kidney and blood. Bradykinin-(1-9) (~100 fmol/g tissue wet weight) and Bradykinin-(1-7) (~70 fmol/g) are the two main bradykinin peptides found in the kidney. Of these, Bradykinin-(1-8) is detectable (~8 fmol/g) and Bradykinin-(4-9) (~12 fmol/g); Bradykinin-(2-9) and Bradykinin-(3-9) are below the detection limit. Bradykinin-(1–9) is only found in blood at extremely low concentrations (~2 fmol/ml), and the detection limit for other bradykinin peptides is exceeded. The angiotensin-converting enzyme (ACE) inhibitor perindopril administered with renal levels of bradykinin-1–8, bradykinin–9, and bradykinin–(1–7) roughly triples as well as reduces correlation. Bradykinin:(1–9) proportion. The aorta, adrenal glands, brain, adrenal glands, heart, and brown adipose tissue can all be treated with N-terminal guided radioimmunoassay. Bradykinin-(1-7) and bradykinin-(1-9) abundances for these tissues are comparable (16-340 fmol/g), although levels of bradykinin-(1-8) are lower[1].

References

[1]. Bradykinin peptides in kidney, blood, and other tissues of the rat. Hypertension. 1993 Feb;21(2):155-65.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~110.62 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (2.77 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.77 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (2.77 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.1062 mL	5.5309 mL	11.0617 mL
	5 mM	0.2212 mL	1.1062 mL	2.2123 mL
	10 mM	0.1106 mL	0.5531 mL	1.1062 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.