Bis-Tris is a widely used buffer.

Physicochemical Properties

Molecular Formula	C8H19NO5
Molecular Weight	209.24
Exact Mass	209.126
CAS #	6976-37-0
Related CAS #	Bis-Tris-d19;352534-93-1
PubChem CID	81462
Appearance	White to off-white solid powder
Density	1.3±0.1 g/cm3
Boiling Point	466.2±40.0 °C at 760 mmHg
Melting Point	104 °C(lit.)
Flash Point	288.0±26.0 °C
Vapour Pressure	0.0±2.6 mmHg at 25°C
Index of Refraction	1.563
LogP	-0.67
Hydrogen Bond Donor Count	5
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	8
Heavy Atom Count	14
Complexity	125
Defined Atom Stereocenter Count	0
InChi Key	OWMVSZAMULFTJU-UHFFFAOYSA-N
InChi Code	InChI=1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
Chemical Name	2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Structure and phosphodiesterase activity of Bis-Tris coordinated lanthanide(III) complexes. [2]. Domain 4 of the anthrax protective antigen maintains structure and binding to the host receptor CMG2 at low pH. Protein Sci. 2009 Nov;18(11):2277-86. [3]. Models of protein modification in Tris-glycine and neutral pH Bis-Tris gels during electrophoresis: effect of gel pH. Anal Biochem. 2005 Jul 15;342(2):237-45.

Additional Infomation

Bis-tris is a buffer substance useful for calibration of glass electrodes and for the preparation of the biochemical and biological buffer solutions; pKa = 6.46 at 25 ℃. It has a role as a buffer. It is functionally related to a member of tris.

Solubility Data

Solubility (In Vitro)	H2O: 250 mg/mL (1194.80 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 50 mg/mL (238.96 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.7792 mL	23.8960 mL	47.7920 mL
	5 mM	0.9558 mL	4.7792 mL	9.5584 mL
	10 mM	0.4779 mL	2.3896 mL	4.7792 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.