Physicochemical Properties
| Molecular Formula | C16H29N5O3S |
| Molecular Weight | 371.4982 |
| Exact Mass | 371.199 |
| CAS # | 109276-34-8 |
| PubChem CID | 83900 |
| Appearance | White to off-white solid powder |
| Density | 1.188 g/cm3 |
| Boiling Point | 777ºC at 760 mmHg |
| Melting Point | 195-196°C |
| Flash Point | 423.7ºC |
| Vapour Pressure | 4.43E-24mmHg at 25°C |
| Index of Refraction | 1.538 |
| LogP | 2.518 |
| Hydrogen Bond Donor Count | 5 |
| Hydrogen Bond Acceptor Count | 5 |
| Rotatable Bond Count | 11 |
| Heavy Atom Count | 25 |
| Complexity | 477 |
| Defined Atom Stereocenter Count | 3 |
| SMILES | C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NN)NC(=O)N2 |
| InChi Key | IJJWOSAXNHWBPR-HUBLWGQQSA-N |
| InChi Code | InChI=1S/C16H29N5O3S/c17-21-14(23)8-2-1-5-9-18-13(22)7-4-3-6-12-15-11(10-25-12)19-16(24)20-15/h11-12,15H,1-10,17H2,(H,18,22)(H,21,23)(H2,19,20,24)/t11-,12-,15-/m0/s1 |
| Chemical Name | 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-(6-hydrazinyl-6-oxohexyl)pentanamide |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | It is common practice to target glycoproteins and glycans using biotin LC hydrazide (BACH). Compared to biotin hydrazide, it is more sensitive and reactive to carbohydrates [1]. Using glutaraldehyde and biotin LC hydrazide (biotin aminocaproyl hydrazide), a novel DNA biotinylation technique was developed [2]. |
| References |
[1]. Quantitation of glycoproteins on electroblots using the biotin-streptavidin complex. Anal Biochem. 1987 May 15;163(1):204-9. [2]. A new biotinylating system for DNA using biotin aminocaproyl hydrazide and glutaraldehyde. Nucleic Acids Res. 1989 Jun 26;17(12):4899-900. |
Solubility Data
| Solubility (In Vitro) | DMSO : ~31 mg/mL (~83.45 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.58 mg/mL (6.94 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.58 mg/mL (6.94 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.58 mg/mL (6.94 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6918 mL | 13.4590 mL | 26.9179 mL | |
| 5 mM | 0.5384 mL | 2.6918 mL | 5.3836 mL | |
| 10 mM | 0.2692 mL | 1.3459 mL | 2.6918 mL |