Bicine is a buffer within the scope of physiological research. Bicine is an analogue of the simple amino acid (AA) glycine.

Physicochemical Properties

Molecular Formula	C6H13NO4
Molecular Weight	163.1717
Exact Mass	163.084
CAS #	150-25-4
Related CAS #	139-41-3 (mono-hydrochloride salt);17123-43-2 (hydrochloride salt)
PubChem CID	8761
Appearance	White to off-white solid powder
Density	1.3±0.1 g/cm3
Boiling Point	388.5±27.0 °C at 760 mmHg
Melting Point	190 °C (dec.)(lit.)
Flash Point	188.8±23.7 °C
Vapour Pressure	0.0±2.0 mmHg at 25°C
Index of Refraction	1.526
LogP	-0.47
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	6
Heavy Atom Count	11
Complexity	111
Defined Atom Stereocenter Count	0
SMILES	O([H])C([H])([H])C([H])([H])N(C([H])([H])C(=O)O[H])C([H])([H])C([H])([H])O[H]
InChi Key	FSVCELGFZIQNCK-UHFFFAOYSA-N
InChi Code	InChI=1S/C6H13NO4/c8-3-1-7(2-4-9)5-6(10)11/h8-9H,1-5H2,(H,10,11)
Chemical Name	2-[bis(2-hydroxyethyl)amino]acetic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Additional Infomation

N,N-bis(2-hydroxyethyl)glycine is a bicine that is a Good's buffer substance, pKa = 8.35 at 20 ℃. It is a conjugate acid of a [bis(2-hydroxyethyl)amino]acetate. It is a tautomer of a [bis(2-hydroxyethyl)ammonio]acetate.

Solubility Data

Solubility (In Vitro)	H2O : ~166.67 mg/mL (~1021.45 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 50 mg/mL (306.43 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.1286 mL	30.6429 mL	61.2858 mL
	5 mM	1.2257 mL	6.1286 mL	12.2572 mL
	10 mM	0.6129 mL	3.0643 mL	6.1286 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.