Benzydamine N-Oxide is a metabolite of Benzydamine. Benzydamine N-Oxide may be utilized to measure flavin-containing monooxygenase activity.

Physicochemical Properties

Molecular Formula	C19H23N3O2
Molecular Weight	325.40
Exact Mass	122.061
CAS #	36504-71-9
PubChem CID	65465
Appearance	Colorless to light yellow ointment
LogP	2.167
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	7
Heavy Atom Count	24
Complexity	385
Defined Atom Stereocenter Count	0
SMILES	C[N+](CCCOC1=NN(CC2C=CC=CC=2)C2=CC=CC=C12)(C)[O-]
InChi Key	VHKKEFPZHPEYJK-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H23N3O2/c1-22(2,23)13-8-14-24-19-17-11-6-7-12-18(17)21(20-19)15-16-9-4-3-5-10-16/h3-7,9-12H,8,13-15H2,1-2H3
Chemical Name	3-(1-benzylindazol-3-yl)oxy-N,N-dimethylpropan-1-amine oxide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Drug metabolite synthesis by immobilized human FMO3 and whole cell catalysts. Microb Cell Fact. 2019 Aug 12;18(1):133. [2]. Benzydamine N-oxygenation as a measure of flavin-containing monooxygenase activity. Methods Mol Biol. 2006;320:157-62.

Solubility Data

Solubility (In Vitro)

DMSO: 50 mg/mL (153.66 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.68 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.68 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (7.68 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.0731 mL	15.3657 mL	30.7314 mL
	5 mM	0.6146 mL	3.0731 mL	6.1463 mL
	10 mM	0.3073 mL	1.5366 mL	3.0731 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.