BODIPY FL L-Cystine is a thiol-reactive green fluorescent dye. BODIPY FL L-Cystine can label membrane proteins, proteins with hydrophobic binding sites, or hydrophobic ligands. (λex=504 nm, λem=511 nm).

Physicochemical Properties

Molecular Formula	C34H36B2N6O6F4S2-2.H+
Molecular Weight	787.44
Exact Mass	788.241
CAS #	1180490-57-6
PubChem CID	168008656
Appearance	Orange to red solid powder
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	14
Rotatable Bond Count	15
Heavy Atom Count	54
Complexity	1670
Defined Atom Stereocenter Count	2
SMILES	C(C1=CC=C2C=C3C(C)=CC(C)=N3[B+3]([F-])([F-])[N-]12)CC(=O)NC(C(=O)[O-])CSSCC(C(=O)[O-])NC(=O)CCC1=CC=C2C=C3C(C)=CC(C)=N3[B+3]([F-])([F-])[N-]12.[H+]
InChi Key	IUOBNSXKYBKQEY-NSOVKSMOSA-N
InChi Code	InChI=1S/C34H38B2F4N6O6S2/c1-19-13-21(3)43-29(19)15-25-7-5-23(45(25)35(43,37)38)9-11-31(47)41-27(33(49)50)17-53-54-18-28(34(51)52)42-32(48)12-10-24-6-8-26-16-30-20(2)14-22(4)44(30)36(39,40)46(24)26/h5-8,13-16,27-28H,9-12,17-18H2,1-4H3,(H,41,47)(H,42,48)(H,49,50)(H,51,52)/t27-,28-/m0/s1
Chemical Name	(2R)-3-[[(2R)-2-carboxy-2-[3-(2,2-difluoro-10,12-dimethyl-1-aza-3-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-3,5,7,9,11-pentaen-4-yl)propanoylamino]ethyl]disulfanyl]-2-[3-(2,2-difluoro-10,12-dimethyl-1-aza-3-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-3,5,7,9,11-pentaen-4-yl)propanoylamino]propanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. An effective thiol-reactive probe for differential scanning fluorimetry with a standard real-time polymerase chain reaction device. Anal Biochem. 2016 Apr 15;499:63-65. [2]. Glutathione-mediated release of Bodipy® from PEG cofunctionalized gold nanoparticles. Int J Nanomedicine. 2012;7:4007-22.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~63.42 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.67 mg/mL (2.12 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.2699 mL	6.3497 mL	12.6994 mL
	5 mM	0.2540 mL	1.2699 mL	2.5399 mL
	10 mM	0.1270 mL	0.6350 mL	1.2699 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.