BODIPY-581/591 NHS ester is a bright red fluorescent dye (excitation: 581 nm; emission: 591 nm). BODIPY-581/591 NHS ester has unique hydrophobic properties that stain lipids, membranes and other lipophilic compounds.

Physicochemical Properties

Molecular Formula	C26H22BN3O4F2
Molecular Weight	489.278
CAS #	150152-70-8
Appearance	Pale purple to purple solid powder
SMILES	[F-][B+3]1(N2=C(C=CC2=CC2=CC=C(CCC(=O)ON3C(CCC3=O)=O)[N-]12)C=CC=CC1C=CC=CC=1)[F-]
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Analyzing the surface of functional nanomaterials-how to quantify the total and derivatizable number of functional groups and ligands. Mikrochim Acta. 2021 Sep 4;188(10):321.

Solubility Data

Solubility (In Vitro)

DMSO : ~25 mg/mL (~51.10 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 1.25 mg/mL (2.55 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0438 mL	10.2191 mL	20.4382 mL
	5 mM	0.4088 mL	2.0438 mL	4.0876 mL
	10 mM	0.2044 mL	1.0219 mL	2.0438 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.