BODIPY505/515 is a BODIPY dye. BODIPY dye is a small molecule dye with strong UV absorption ability. Its fluorescence peak is relatively sharp and its quantum yield is high. It is relatively insensitive to the polarity and pH of the environment and, therefore, is relatively stable under different physiological conditions. Due to the asymmetry of its structure, BODIPY has a variety of analogue structural products. BODIPY lipid droplet dye can penetrate the cell membrane well into the interior of the cell and perform specific staining by localizing on the polar lipids in the cell. Therefore, the dye may be utilized to label living cells and fixed cells. excitation and emmission maximum (wavelength): 505/515 nm.

Physicochemical Properties

Molecular Formula	C13H15BF2N2
Molecular Weight	248.0794
Exact Mass	248.129
CAS #	21658-70-8
PubChem CID	16679984
Appearance	Brown to red solid powder
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	0
Heavy Atom Count	18
Complexity	468
Defined Atom Stereocenter Count	0
SMILES	F[B-]1(N2C(C([H])([H])[H])=C([H])C(C([H])([H])[H])=C2C([H])=C2C(C([H])([H])[H])=C([H])C(C([H])([H])[H])=[N+]21)F
InChi Key	TWYZTUZOEQTCOO-UHFFFAOYSA-N
InChi Code	InChI=1S/C13H15BF2N2/c1-8-5-10(3)17-12(8)7-13-9(2)6-11(4)18(13)14(17,15)16/h5-7H,1-4H3
Chemical Name	2,2-difluoro-4,6,10,12-tetramethyl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaene
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

Guidelines for use 1. Working solution for BODIPY505/515 stock solution 1.1 Get the stock solution ready. 382 μL of anhydrous DMSO should be loaded with 1 milligram of BODIPY505/515. Take out the 10 mM reference solution. Note: It is advised to prepare a 1–10 μM BODIPY505/515 working solution by either aliquoting the BODIPY505/515 storage solution at -20°C or using pre-made serum-free cell culture medium or PBS filling solution with the 1.2 working solution. Note: Please keep BODIPY505 at -80°C away from light and adjust to real conditions. 2. Cell staining (suspended cells) 2.1 Centrifuge the cells, add PBS, and wash twice for five minutes each time. Following a centrifugation for 3–4 minutes and the removal of the supernatant, add 1 mL BODIPY505/515 2.3 400 g when the cell density reaches 1×106/mL 2.2. 2.4 Wash the cells twice, for five minutes each time, with PBS added. 2.5 Resuspend in 1 milliliter of PBS 3 or serum-free water. Staining of adherent cells 3.1 The adherent cells are cultured on sterile coverslips. 3.2 Aspirate extra culture after removing the coverslip from the cells. 3.3 After adding 100 μL of the dye working solution, fully cover the cells with your hand waves. It will require between five and thirty minutes to complete. 3.4 Aspirate the dye working solution, use a fluorescence microscope or base, and wash it twice, for a duration of five minutes each, in culture media. using flow cytometry observation. Conditions for storage: Keep away from light and store at -20°C for a year. Notes 1. Kindly modify the BODIPY505/515 working fluid concentration and wastewater time to reflect the current circumstances. 2. In the experiment, it is advised to use laboratory control. Before doing following tests, incubate the document cells with the cells in a gradient of 30 μM oleic acid for 8 hours. 3. This product may not be used in food or medication, nor may it be utilized for clinical diagnosis or treatment. It is intended solely for professional use in scientific study. 4. Please use a lab coat and disposable gloves for your health and safety.

References

[1]. Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining. Bioresour Technol. 2013 Jan;127:386-90. [2]. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912.

Solubility Data

Solubility (In Vitro)

DMSO : ~12.5 mg/mL (~50.39 mM)

Solubility (In Vivo)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.0310 mL	20.1548 mL	40.3096 mL
	5 mM	0.8062 mL	4.0310 mL	8.0619 mL
	10 mM	0.4031 mL	2.0155 mL	4.0310 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.