BODIPY493/503 methyl bromide is a BODIPY dye. BODIPY dye is a small molecule dye with strong UV absorption ability. Its fluorescence peak is relatively sharp and its quantum yield is high. It is relatively insensitive to the polarity and pH of the environment and, therefore, is relatively stable under different physiological conditions. Due to the asymmetry of its structure, BODIPY has a variety of analogue structural products. BODIPY lipid droplet dye can penetrate the cell membrane well into the interior of the cell and perform specific staining by localizing on the polar lipids in the cell. Therefore, the dye may be utilized to label living cells and fixed cells. excitation and emmission maximum (wavelength): 493/503 nm.

Physicochemical Properties

Molecular Formula	C14H16BN2F2BR
Molecular Weight	341.00204
Exact Mass	340.055
CAS #	216434-81-0
PubChem CID	71774308
Appearance	Brown to reddish brown solid powder
LogP	3.201
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	1
Heavy Atom Count	20
Complexity	525
Defined Atom Stereocenter Count	0
InChi Key	JLSHPOSUMKVMAP-UHFFFAOYSA-N
InChi Code	InChI=1S/C14H16BBrF2N2/c1-8-5-10(3)19-13(8)12(7-16)14-9(2)6-11(4)20(14)15(19,17)18/h5-6H,7H2,1-4H3
Chemical Name	8-(bromomethyl)-2,2-difluoro-4,6,10,12-tetramethyl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaene
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

Guidelines for usage 1. BODIPY493/503 methyl bromide working solution composition 1.1 Prepare the stock solution by adding 1 milligram of BODIPY493/503 methyl bromide to 10 mM of anhydrous DMSO (382 μL). Note: To generate a 1-10 μM BODIPY493/503 methyl bromide working solution, it is advised to aliquot the BODIPY493/503 methyl bromide storage solution into the 1.2 working solution rather than using pre-prepared serum-free cell culture medium or PBS. Please prepare the BODIPY493/503 methyl bromide working solution for usage by adjusting its concentration to the current circumstances. 2. Suspended cell staining (2.1): Centrifuge cells, add PBS, then wash twice for five minutes each time. 2.2 Add 1 mL of BODIPY493/503 methyl bromide working solution and stir for 5–30 minutes when the cell density is 1×106/mL. 2.3 Centrifuge at 400g for 3–4 minutes and discard the supernatant. 2.4 After adding PBS, wash the cells twice for five minutes each. 2.5 Re-suspend the cells in 1 milliliter of PBS or serum-free media, and use a flow cytometer or fluorescence microscope to observe. 3. Adherent cell staining: 3.1 Grow the adherent cells on sterile coverslips. 3.2 Cover the cells fully with 100 μL of the dye working solution from step 3.3, give them a gentle shake, and sterilize for a duration of 5 to 30 minutes. 3.4 Aspirate the dye working solution, rinse with culture medium two or three times for five minutes each, and use a flow cytometer or fluorescence microscope to monitor. Conditions for storage: Keep for a year at -20°C and out of direct sunlight. Observations 1. Kindly modify the BODIPY493/503 methyl bromide working solution concentration and the left-hand time interval based on the current circumstances. 2. It is advised that the experiment include an acute control. Before doing any further tests, the cells need to be treated to a 30 μM oleic acid carrier for 8 hours. 3. This product is not intended for use in clinical diagnosis or treatment; rather, it should only be used by experts conducting scientific research. 4. When working, please wear immediate gloves and a lab coat for your health and safety.

References

[1]. Synthesis and spectral properties of cholesterol- and FTY720-containing boron dipyrromethene dyes. Synthesis and spectral properties of cholesterol- and FTY720-containing boron dipyrromethene dyes. J Org Chem. 2007 Oct 26;72(22):8376-82. [2]. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912.

Solubility Data

Solubility (In Vitro)

DMSO : ~10 mg/mL (~29.33 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 1 mg/mL (2.93 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9326 mL	14.6628 mL	29.3255 mL
	5 mM	0.5865 mL	2.9326 mL	5.8651 mL
	10 mM	0.2933 mL	1.4663 mL	2.9326 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.