BNN3 is a caged nitric oxide (NO) donor. BNN3 is membrane permeable/penetrable and incorporated into lipid membranes.

Physicochemical Properties

Molecular Formula	C8H10N4O2
Molecular Weight	194.19
Exact Mass	194.08
CAS #	6947-38-2
PubChem CID	245218
Appearance	Light yellow to yellow solid powder
Density	1.23g/cm3
Boiling Point	430.9ºC at 760 mmHg
Flash Point	214.4ºC
Index of Refraction	1.58
LogP	1.921
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	2
Heavy Atom Count	14
Complexity	182
Defined Atom Stereocenter Count	0
SMILES	CN(c1ccc(cc1)N(C)N=O)N=O
InChi Key	WKDYJAMEXVFEEA-UHFFFAOYSA-N
InChi Code	InChI=1S/C8H10N4O2/c1-11(9-13)7-3-5-8(6-4-7)12(2)10-14/h3-6H,1-2H3
Chemical Name	N-methyl-N-[4-[methyl(nitroso)amino]phenyl]nitrous amide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	BNN3 produces NO, λEm (max): ~300 nm, using a laser beam or xenon lamp[1].
References	[1]. Namiki S, et, al. Bis-N-nitroso-caged nitric oxides: photochemistry and biological performance test by rat aorta vasorelaxation. Bioorg Med Chem. 1999 Aug;7(8):1695-702.

Solubility Data

Solubility (In Vitro)

DMSO: 50 mg/mL (257.48 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.25 mg/mL (6.44 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.1496 mL	25.7480 mL	51.4960 mL
	5 mM	1.0299 mL	5.1496 mL	10.2992 mL
	10 mM	0.5150 mL	2.5748 mL	5.1496 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.