BMVC2 (o-BMVC) is a disubstituted carbazole analogue of BMVC and a G-quadruplex (G4) stabilizer.

Physicochemical Properties

Molecular Formula	C28H25I2N3
Molecular Weight	657.327151060104
Exact Mass	657.013
CAS #	850559-51-2
PubChem CID	11377294
Appearance	Yellow to orange solid powder
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	4
Heavy Atom Count	33
Complexity	588
Defined Atom Stereocenter Count	0
SMILES	[I-].[I-].N1C2C=CC(/C=C/C3C=CC=C[N+]=3C)=CC=2C2C=C(/C=C/C3C=CC=C[N+]=3C)C=CC1=2
InChi Key	KSHYRGPLGVYWRF-UHFFFAOYSA-M
InChi Code	InChI=1S/C28H24N3.2HI/c1-30-17-5-3-7-23(30)13-9-21-11-15-27-25(19-21)26-20-22(12-16-28(26)29-27)10-14-24-8-4-6-18-31(24)2;;/h3-20H,1-2H3;2*1H/q+1;;/p-1
Chemical Name	3,6-bis[(E)-2-(1-methylpyridin-1-ium-2-yl)ethenyl]-9H-carbazole;diiodide
Synonyms	BMVC 2; BMVC-2
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	After adding Cu2+, screen BMVC2 by observing the elimination of the d[AG3(T2AG3)3] (HT22) 291 nm CD signal. A better G4 stabilizer will be determined by a longer unfolding time. Two time constants that are comparable to the unfolding parameters of BMVC [1]—133.7 s (29%) and 2816 s (71%)—can be used to fit the unfolding curve of HT22 in the presence of BMVC2.
References	[1]. A Novel Method for Screening G-quadruplex Stabilizers to Human Telomeres. Journal of the Chinese Chemical Society, 2011, 58, 296-300.

Solubility Data

Solubility (In Vitro)

DMSO : ~16.67 mg/mL (~25.36 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.67 mg/mL (2.54 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.5213 mL	7.6065 mL	15.2131 mL
	5 mM	0.3043 mL	1.5213 mL	3.0426 mL
	10 mM	0.1521 mL	0.7607 mL	1.5213 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.