BM-962 (Compound 31) is a potent small molecule inhibitor with IC50 values of 4 nM (Ki=0.8 nM) for Bcl-2 and 3.9 nM (Ki <1 nM) for Bcl-xL. BM-962 has inhibitory effects on H1417 and H146 cell lines with IC50 values of 9 and 13 nM, respectively. BM-962 has potential for use in cancer research.

Physicochemical Properties

Molecular Formula	C53H58CLF3N6O7S3
Molecular Weight	1079.71
CAS #	1391107-57-5
Appearance	White to off-white solid powder
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Structure-based discovery of BM-957 as a potent small-molecule inhibitor of Bcl-2 and Bcl-xL capable of achieving complete tumor regression. J Med Chem. 2012 Oct 11;55(19):8502-14. [2]. Bcl-2/Bcl-xL inhibitors and therapeutic methods using the same. US20120189539. 2012 July 26.

Solubility Data

Solubility (In Vitro)

DMSO : ~200 mg/mL (~185.23 mM; with ultrasonication)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 5 mg/mL (4.63 mM)(saturation unknown) in 10% DMSO 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution and add it to 900 μL corn oil and mix well.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.9262 mL	4.6309 mL	9.2617 mL
	5 mM	0.1852 mL	0.9262 mL	1.8523 mL
	10 mM	0.0926 mL	0.4631 mL	0.9262 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.