BCA is the disodium salt of 2,2-Biquinoline-4,4-dicarboxylic acid, which can be used for the analysis and determination of Cu and protein.

Physicochemical Properties

Molecular Formula	C20H10N2NA2O4
Molecular Weight	388.2839
Exact Mass	388.043
CAS #	979-88-4
PubChem CID	164763
Appearance	White to light yellow solid powder
Boiling Point	530.6ºC at 760 mmHg
Flash Point	274.7ºC
LogP	1.177
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	1
Heavy Atom Count	28
Complexity	495
Defined Atom Stereocenter Count	0
InChi Key	AUPXFICLXPLHBB-UHFFFAOYSA-L
InChi Code	InChI=1S/C20H12N2O4.2Na/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18;;/h1-10H,(H,23,24)(H,25,26);;/q;2*+1/p-2
Chemical Name	disodium;2-(4-carboxylatoquinolin-2-yl)quinoline-4-carboxylate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage.(2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Tripeptide GGH as the inhibitor of copper-amyloid-β-mediated redox reaction and toxicity . ACS Chemical Neuroscience, 2016, 7(9): 1255-1263. [2]. Protein quantification by bicinchoninic acid (BCA) assay follows complex kinetics and can be performed at short incubation times . Analytical biochemistry, 2020, 608: 113904. [3]. The colorimetric determination of copper in wine: total copper . Australian Journal of Grape and Wine Research, 2020, 26(2): 121-129.

Solubility Data

Solubility (In Vitro)	H2O : ~100 mg/mL (~257.55 mM) DMSO : ~2 mg/mL (~5.15 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 25 mg/mL (64.39 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.5755 mL	12.8773 mL	25.7546 mL
	5 mM	0.5151 mL	2.5755 mL	5.1509 mL
	10 mM	0.2575 mL	1.2877 mL	2.5755 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.