Physicochemical Properties
| Molecular Formula | C47H48BCLF2N6O5 |
| Molecular Weight | 861.182037353516 |
| CAS # | 2456476-47-2 |
| Appearance | Orange to red solid powder |
| SMILES | CC1C=C(C)N2[B-](F)(F)[N+]3=C(C)C=C(C)C3=C(C3C=CC(OCCCCCCN4N=NC(COC5C(=CC=C6C=C7C8=CC9OCOC=9C=C8CC[N+]7=CC=56)OC)=C4)=CC=3)C=12.[Cl-] |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | The antiproliferative action of BBR-BODIPY (1-32 μM; 24 hours; MCF7 and MDA-MB-231 cells) has been shown [1]. MCF7 cells can absorb BBR-BODIPY (2-16 μM; 5-60 minutes) quickly in less than 5 minutes, and the intensity of the fluorescence varies with time [1]. BBR-BODIPY (1-32 μM; 24 hours; MCF7 and MDA-MB-231 cells) accumulates in the mitochondria and shows yellow fluorescence with a Pearson colocalization coefficient of 0.72 when it overlaps with Mito Tracker Red [1]. Through the mitochondrial route, BBR-BODIPY (8 μM, 12 hours) causes apoptosis in MCF7 cells [1]. |
| Cell Assay |
Cytotoxicity assay[1] Cell Types: MCF7 and MDA-MB-231 Cell Tested Concentrations: 1, 2, 4, 8, 16 and 32 μM Incubation Duration: 24 hrs (hours) Experimental Results: MCF7 and MDA-MB-231 cells were inhibited, IC50 The values are 40.81 and 41.46 μM for MDA-MB-231 cells respectively. Western Blot Analysis [1] Cell Types: MCF7 cells Tested Concentrations: 8 μM Incubation Duration: 12 hrs (hours) Experimental Results: The amounts of Bax and Cyto C released from mitochondria and cleaved Caspase 9 were up-regulated in MCF7 cells. |
| References |
[1]. Synthesis of a novel fluorescent berberine derivative convenient for its subcellular localization study. Bioorg Chem. 2020 Aug;101:104021. |
Solubility Data
| Solubility (In Vitro) | May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.1612 mL | 5.8060 mL | 11.6120 mL | |
| 5 mM | 0.2322 mL | 1.1612 mL | 2.3224 mL | |
| 10 mM | 0.1161 mL | 0.5806 mL | 1.1612 mL |