BAY-298 is an orally bioactive and selective luteinizing hormone receptor (LH-R) antagonist (inhibitor) with IC50 for hLH (human LH), rLH (rat LH) and cLH (cynomolgus monkey LH), respectively. are 96 nM, 23 nM and 78 nM. BAY-298 can reduce sex hormone levels.

Physicochemical Properties

Molecular Formula	C27H21CLFN3O2
Molecular Weight	473.93
Exact Mass	473.13
CAS #	2471978-97-7
PubChem CID	139600337
Appearance	Off-white to light yellow solid powder
LogP	5.6
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	4
Heavy Atom Count	34
Complexity	660
Defined Atom Stereocenter Count	1
SMILES	C1C=C([C@@H]2N(CCC3=C2C=CC=N3)C(NC2=CC=C(C=C2)OC2C=CC(=CC=2)F)=O)C=CC=1Cl
InChi Key	FMQWOAKTQPLQBR-SANMLTNESA-N
InChi Code	InChI=1S/C27H21ClFN3O2/c28-19-5-3-18(4-6-19)26-24-2-1-16-30-25(24)15-17-32(26)27(33)31-21-9-13-23(14-10-21)34-22-11-7-20(29)8-12-22/h1-14,16,26H,15,17H2,(H,31,33)/t26-/m0/s1
Chemical Name	(5S)-5-(4-chlorophenyl)-N-[4-(4-fluorophenoxy)phenyl]-7,8-dihydro-5H-1,6-naphthyridine-6-carboxamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: 185 nM (hLH), 46nM (rLH) and 78 nM (cLH)[1]
ln Vivo	Serum estradiol levels in proestrus are dose-dependently lowered by BAY-298 (oral; 4.5-72 mg/kg/day; for 8 days)[1]. The half-lives of BAY-298 (iv at 0.5 mg/kg or po at 2 mg/kg) are 31 and 33 hours, respectively. For iv and po, the Cmaxs are 0.28 kg/L and 0.066 kg/L, respectively[1].
Animal Protocol	Animal/Disease Models: Intact female rats[1] Doses: 4.5, 9, 18 , 36, 72 mg/kg Route of Administration: Oral; for 8 days Experimental Results: Dosedependently lowered serum estradiol levels in proestrus. Animal/Disease Models: Female and male Wistar rats[1] Doses: 0.5 mg/kg of iv or 2 mg/kg of po Route of Administration: Iv or po Experimental Results: Has t1/2s of 31 hrs (hours) and 33 hrs (hours) for iv and po. And the Cmaxs are 0.28 kg/L and 0.066 kg/L for iv and po.
References	[1]. Discovery of BAY-298 and BAY-899: Tetrahydro-1,6-naphthyridine-Based, Potent and Selective Antagonists of the Luteinizing Hormone Receptor Which Reduce Sex Hormone Levels In Vivo. J Med Chem. 2019 Oct 31.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (211.00 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (5.28 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1100 mL	10.5501 mL	21.1002 mL
	5 mM	0.4220 mL	2.1100 mL	4.2200 mL
	10 mM	0.2110 mL	1.0550 mL	2.1100 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.