Azaspirene ((-)-Azaspirene) is an angiogenesis and Raf-1 activation inhibitor isolated from the fungus Neosartorya sp. Azaspirene inhibits vascular endothelial growth factor (VEGF)-induced human umbilical vein endothelial cell (HUVEC) migration and Raf-1 activation, but has no effect on the activation of receptor containing kinase insert domain/fetal liver kinase 1 (VEGF receptor 2).

Physicochemical Properties

Molecular Formula	C21H23NO5
Molecular Weight	369.41
CAS #	461644-34-8
SMILES	O1C(/C=C/C=C/CC)=C(C)C([C@]21C(N[C@@](CC1C=CC=CC=1)([C@@H]2O)O)=O)=O
Synonyms	(-)-Azaspirene
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Azaspirene, a fungal product, inhibits angiogenesis by blocking Raf-1 activation. Cancer Sci. 2008 Sep;99(9):1853-8.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7070 mL	13.5351 mL	27.0702 mL
	5 mM	0.5414 mL	2.7070 mL	5.4140 mL
	10 mM	0.2707 mL	1.3535 mL	2.7070 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.