Autophagy inducer 7 (Compound SSA) is an inducer of autophagy and apoptosis. Autophagy inducer 7 activates autophagy by inhibiting Akt/mTOR signaling and the expression of downstream proteins. Autophagy inducer 7 inhibits DNA synthesis and causes G0-G1 cell cycle arrest. Autophagy inducer 7 inhibits tumor cell growth.

Physicochemical Properties

Molecular Formula	C24H27FN2OS
Molecular Weight	410.55
CAS #	944159-20-0
Appearance	Solid powder
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Autophagy inducer 7 (72 h) inhibits the growth of human lung adenocarcinoma cell lines with IC50s of 1.95 μM (HOP-62), 4.72 μM (A549), and 3.05 μM (H1299)[1]. Autophagy inducer 7 (0-10 μM, 24 h) induced apoptosis in HOP-62, A549, and H1299 cells, but not as significantly as Sulindac sulfide and Staurosporine [1]. Autophagy inducer 7 (0-7.5 μM, 24 h) inhibits DNA synthesis in HOP-62, A549, and H1299 cells and increases the percentage of cells in the G0-G1 phase of the cell cycle [1]. Autophagy inducer 7 (0-7.5 μM, 24 h) induces autophagy and increases autophagic flux by inhibiting Akt/mTOR/p70S6k signaling in A549 cells [1].
Cell Assay	Apoptosis Analysis[1] Cell Types: HOP-62, A549, H1299 cells Tested Tested Concentrations: 2.5, 5, 7.5, 10 μM Incubation Duration: 24 h Experimental Results: Induced caspase3/7 activation dose-dependently. Induced cleaved PARP expression at 10 μM. Increased Annexin-V surface staining and PI labeling. Cell Autophagy Assay[1] Cell Types: HOP-62, A549, H1299 cells Tested Tested Concentrations: 2.5, 5, 7.5, 10 μM Incubation Duration: 24 h Experimental Results: Induced dose- and time-dependent cleavage of cytosolic LC3-I to autophagosome-associated LC3-II. Decreased p62 expression. Displayed a striking accumulation of vesicle-like structures within the cytoplasm. Increased both the number and size of acidic vesicles within the cytoplasm.
References	[1]. A novel sulindac derivative inhibits lung adenocarcinoma cell growth through suppression of Akt/mTOR signaling and induction of autophagy. Mol Cancer Ther. 2013 May;12(5):663-74.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~121.79 mM; with ultrasonication)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.4358 mL	12.1788 mL	24.3576 mL
	5 mM	0.4872 mL	2.4358 mL	4.8715 mL
	10 mM	0.2436 mL	1.2179 mL	2.4358 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.