Araliadiol is a polyacetylenic compound extracted from the leaves of Aralia cordata Thunb. Araliadiol inhibits MCF-7 cell growth with IC50 of 6.41 µg/mL.

Physicochemical Properties

Molecular Formula	C15H20O2
Molecular Weight	232.33
Exact Mass	232.146
CAS #	1354638-93-9
PubChem CID	124355647
Appearance	Typically exists as solid at room temperature
Density	1.0±0.1 g/cm3
Boiling Point	381.2±42.0 °C at 760 mmHg
Flash Point	176.1±22.5 °C
Vapour Pressure	0.0±2.0 mmHg at 25°C
Index of Refraction	1.531
LogP	5.26
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	7
Heavy Atom Count	17
Complexity	367
Defined Atom Stereocenter Count	2
SMILES	CCCCC/C=C\[C@H](C#CC#C[C@H](C=C)O)O
InChi Key	KZRIUXSFFOWKRO-USJBSMOHSA-N
InChi Code	InChI=1S/C15H20O2/c1-3-5-6-7-8-12-15(17)13-10-9-11-14(16)4-2/h4,8,12,14-17H,2-3,5-7H2,1H3/b12-8-/t14-,15+/m0/s1
Chemical Name	(3S,8R,9Z)-pentadeca-1,9-dien-4,6-diyne-3,8-diol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Treatment with Araliadiol (20-80 µM; 0-48 hours; MCF-7 cells) slows the advancement of the MCF-7 cell cycle at the G1-S transition [1]. Treatment of MCF-7 cells with araliadiol (20-80 µM; 0-48 hours) suppresses the phosphorylation of retinoblastoma protein (Rb) and results in lower levels and increased expression of cdk4 p21 (WAF-1/Cip1) and cyclin D3[1].
Cell Assay	Cell cycle analysis[1] Cell Types: MCF-7 Cell Tested Concentrations: 20 µM, 40 µM and 80 µM Incubation Duration: 0 hrs (hours), 6 hrs (hours), 12 hrs (hours), 18 hrs (hours), 24 hrs (hours) and 48 hrs (hours) Experimental Results: In G1 phase of the cell cycle of cells increased in a dose-dependent manner (from 54.7% to 72.0%). Western Blot Analysis[1] Cell Types: MCF-7 Cell Tested Concentrations: 20 µM, 40 µM and 80 µM Incubation Duration: 48 hrs (hours) Experimental Results: Phosphorylation of retinoblastoma protein (Rb) was inhibited in MCF-7 cells while Phosphorylation levels diminished cyclin D3 and cyclin-dependent kinase 4 (cdk4) levels and increased p21 (WAF-1/Cip1) expression.
References	[1]. Inhibitory effect of human breast cancer cell proliferation via p21-mediated G1 cell cycle arrest by araliadiol isolated from Aralia cordata Thunb. Planta Med. 2011 Jan;77(2):164-8.
Additional Infomation	Araliadiol has been reported in Aralia cordata with data available.

Solubility Data

Solubility (In Vitro)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

Solubility (In Vivo)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.3042 mL	21.5211 mL	43.0422 mL
	5 mM	0.8608 mL	4.3042 mL	8.6084 mL
	10 mM	0.4304 mL	2.1521 mL	4.3042 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.