Arachidonic acid (Icosanoic acid) is a long-chain fatty acid found in all mammalian cells, often esterified into membrane phospholipids, and is one of the most abundant polyunsaturated fatty acids in human tissues.

Physicochemical Properties

Molecular Formula	C20H40O2
Molecular Weight	312.5304
Exact Mass	312.302
CAS #	506-30-9
Related CAS #	Arachidic acid-d2;232600-70-3;Arachidic acid-d39;39756-32-6;Arachidic acid-d3;202480-70-4;Arachidic acid-13C;335080-96-1;Arachidic acid-d4;1219803-69-6;Arachidic acid-d4-1;2483831-15-6
PubChem CID	10467
Appearance	White to off-white solid powder
Density	0.9±0.1 g/cm3
Boiling Point	376.4±5.0 °C at 760 mmHg
Melting Point	74-76 °C(lit.)
Flash Point	169.7±12.5 °C
Vapour Pressure	0.0±0.9 mmHg at 25°C
Index of Refraction	1.457
LogP	9.28
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	18
Heavy Atom Count	22
Complexity	226
Defined Atom Stereocenter Count	0
InChi Key	VKOBVWXKNCXXDE-UHFFFAOYSA-N
InChi Code	InChI=1S/C20H40O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20(21)22/h2-19H2,1H3,(H,21,22)
Chemical Name	icosanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Analysis of fatty acid composition of anaerobic rumen fungi. Folia Microbiol (Praha). 2008;53(3):217-20. [2]. The discovery and early structural studies of arachidonic acid. J Lipid Res. 2016 Jul;57(7):1126-32. [3]. Arachidonic acid cytotoxicity in leukocytes: implications of oxidative stress and eicosanoid synthesis. Biol Cell. 2002 Sep;94(4-5):251-65. [4]. Effect of long-term administration of arachidonic acid on n-3 fatty acid deficient mice. Prostaglandins Leukot Essent Fatty Acids. 2015 Apr;95:41-5.

Additional Infomation

Icosanoic acid is a C20 striaght-chain saturated fatty acid which forms a minor constituent of peanut (L. arachis) and corn oils. Used as an organic thin film in the production of liquid crystals for a wide variety of technical applications. It has a role as a plant metabolite. It is a straight-chain saturated fatty acid and a long-chain fatty acid. It is a conjugate acid of an icosanoate. Arachidic acid has been reported in Calodendrum capense, Amaranthus hybridus, and other organisms with data available. Arachidic Acid is a saturated long-chain fatty acid with a 20-carbon backbone. Arachidic acid is found naturally as a minor component of peanut oil. - Arachidic acid (C20:0, a saturated long-chain fatty acid) was detected in the fatty acid composition analysis of anaerobic rumen fungi (e.g., Neocallimastix frontalis , Piromyces communis ) in [1]. Its relative content accounted for 2.1%-3.5% of the total fatty acids in the tested fungal strains, lower than that of unsaturated fatty acids (e.g., linoleic acid, oleic acid) [1] - Literatures [2], [3], and [4] focused on arachidonic acid (C20:4, an unsaturated polyunsaturated fatty acid), which is a different compound from Arachidic acid (C20:0). - Arachidic acid is naturally present in small amounts in some plant oils (e.g., peanut oil, palm oil) and animal fats [1]

Solubility Data

Solubility (In Vitro)

DMSO : ~4.76 mg/mL (~15.23 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1 mg/mL (3.20 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1 mg/mL (3.20 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1 mg/mL (3.20 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.1997 mL	15.9985 mL	31.9969 mL
	5 mM	0.6399 mL	3.1997 mL	6.3994 mL
	10 mM	0.3200 mL	1.5998 mL	3.1997 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.