Apoptosis inducer 34 (Compound 4) is a small molecule compound that induces apoptosis by directly activating the basal apoptotic machinery. Apoptosis inducer 34 promotes the oligomerization of Apaf-1 to form mature apoptotic bodies, thereby activating caspase-9 and caspase-3. Apoptosis inducer 34 significantly activates the apoptotic pathway in Jurkat cells by enhancing the cytochrome c-dependent apoptotic signaling pathway and induces PARP cleavage and chromosomal DNA fragmentation. In addition, Apoptosis inducer 34 has low toxicity to normal cells and shows the potential to selectively target cancer cells. Apoptosis inducer 34 is expected to be used in cancer research related to the apoptotic pathway.

Physicochemical Properties

Molecular Formula	C17H15CL2NO2
Molecular Weight	336.21
CAS #	582314-48-5
SMILES	ClC=1C=CC(=CC1Cl)CNC(O[C@H]2CCC3=CC=CC=C32)=O
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7533-8.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9743 mL	14.8717 mL	29.7433 mL
	5 mM	0.5949 mL	2.9743 mL	5.9487 mL
	10 mM	0.2974 mL	1.4872 mL	2.9743 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.