Antiproliferative agent-64 (Compound 76) is an inhibitor of eukaryotic translation initiation factor 4E (eIF4E), which blocks the secondary structure of mRNA, thereby inhibiting protein translation. Antiproliferative agent-64 inhibits the 5' untranslated region of c-Myc (c-myc 5'UTR) with an EC50 of 1.2 nM and inhibits the 5'UTR encoding tubulin (tub 5'UTR) with an EC50 of 40 nM. Antiproliferative agent-64 inhibits the proliferation of MDA-MB-231 with an EC50 of 7 nM.

Physicochemical Properties

Molecular Formula	C29H28N2O6
Molecular Weight	500.54
CAS #	1139263-21-0
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Synthetic silvestrol analogues as potent and selective protein synthesis inhibitors. J Med Chem. 2012 Oct 25;55(20):8859-78.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9978 mL	9.9892 mL	19.9784 mL
	5 mM	0.3996 mL	1.9978 mL	3.9957 mL
	10 mM	0.1998 mL	0.9989 mL	1.9978 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.