antifungal compound/agent 1 is an effective antifungal compound/agent.

Physicochemical Properties

Molecular Formula	C19H13CLN2O3
Molecular Weight	352.77112364769
Exact Mass	352.061
CAS #	1265166-14-0
PubChem CID	131704488
Appearance	Light brown to black solid powder
LogP	3.9
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	2
Heavy Atom Count	25
Complexity	600
Defined Atom Stereocenter Count	0
InChi Key	QUAFTMJUVQDZHE-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H13ClN2O3/c1-22-15-7-6-12(23)8-13(15)17-18(22)16(24)9-14(19(17)25)21-11-4-2-10(20)3-5-11/h2-9,21,23H,1H3
Chemical Name	3-(4-chloroanilino)-6-hydroxy-9-methylcarbazole-1,4-dione
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Antifungal agent 1 had an effective inhibitory impact on Candida albicans, Candida tropicalis, Candida krusei, and Candida neoformans, with MIC values of 6.3, 25.0, 12.5, and 25.0 μg/mL respectively [1].
References	[1]. Ryu CK, et al. Synthesis and antifungal evaluation of 6-hydroxy-1H-carbazole-1,4(9H)-diones. Bioorg Med Chem Lett. 2011 Jan 1;21(1):427-30.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~283.47 mM） H2O : < 0.1 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (7.09 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (7.09 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.8347 mL	14.1735 mL	28.3471 mL
	5 mM	0.5669 mL	2.8347 mL	5.6694 mL
	10 mM	0.2835 mL	1.4174 mL	2.8347 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.