Anti-inflammatory agent 38 (compound 23d) is a potent Nrf2/HO-1 inhibitor (antagonist) with IC50 of 0.38 μM for NO. Anti-inflammatory agent 38 can significantly reduce ROS levels in cells. Anti-inflammatory agent 38 may be utilized in anti-inflammatory research.

Physicochemical Properties

Molecular Formula	C36H46N2O13S
Molecular Weight	746.82
CAS #	3032633-42-1
Appearance	White to off-white solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	IC50: 0.38 μM (NO)[1]
ln Vitro	In RAW264.7 cells stimulated by LPS (0.5 µg/mL LPS stimulates for 12 hours), anti-inflammatory agent 38 (compound 23d)(0.25, 0.5, 1 and 2 µM; 3 hours) can decrease ROS production[1]. Anti-inflammatory drug 38 (0.25, 0.5, 1 and 2 µM; 3 hours) promotes LPS-induced Nrf2 nuclear translocation and dose-dependently upregulates Nrf2 and HO-1 expression in LPS-stimulated RAW264.7 cells.[1]
Cell Assay	Western Blot Analysis Cell Types: RAW264.7(0.5 µg/mL LPS stimulates for 12 hrs (hours))[1] Tested Concentrations: 0.25, 0.5, 1 and 2 µM Incubation Duration: 3 hrs (hours) Experimental Results: Dramatically increased the expression of Nrf2 and HO-1 in a dose-dependent manner.
References	[1]. Synthesis of lathyrane diterpenoid nitrogen-containing heterocyclic derivatives and evaluation of their anti-inflammatory activities. Bioorg Med Chem.

Solubility Data

Solubility (In Vitro)

DMSO :~100 mg/mL (~133.90 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 5 mg/mL (6.70 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.3390 mL	6.6951 mL	13.3901 mL
	5 mM	0.2678 mL	1.3390 mL	2.6780 mL
	10 mM	0.1339 mL	0.6695 mL	1.3390 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.