Beta-Amyloid (25-35) (Amyloid beta-peptide (25-35)) is the Aβ(25-35) fragment of Alzheimer's amyloid beta peptide and displays neurotoxic activity in cultured cells.

Physicochemical Properties

Molecular Formula	C45H81N13O14S
Molecular Weight	1060.2683
Exact Mass	1059.57
CAS #	131602-53-4
PubChem CID	3407255
Appearance	White to off-white solid powder
Density	1.249g/cm3
Boiling Point	1517.336ºC at 760 mmHg
Flash Point	871.453ºC
Vapour Pressure	0mmHg at 25°C
Index of Refraction	1.544
LogP	1.058
Hydrogen Bond Donor Count	15
Hydrogen Bond Acceptor Count	17
Rotatable Bond Count	37
Heavy Atom Count	73
Complexity	1880
Defined Atom Stereocenter Count	0
InChi Key	WIHBNMPFWRHGDF-UHFFFAOYSA-N
InChi Code	InChI=1S/C45H81N13O14S/c1-9-24(5)36(43(69)50-21-35(63)52-29(17-23(3)4)40(66)55-28(45(71)72)14-16-73-8)58-44(70)37(25(6)10-2)57-38(64)26(7)51-34(62)20-49-39(65)27(13-11-12-15-46)54-41(67)30(18-32(48)60)56-42(68)31(22-59)53-33(61)19-47/h23-31,36-37,59H,9-22,46-47H2,1-8H3,(H2,48,60)(H,49,65)(H,50,69)(H,51,62)(H,52,63)(H,53,61)(H,54,67)(H,55,66)(H,56,68)(H,57,64)(H,58,70)(H,71,72)
Chemical Name	2-[[2-[[2-[[2-[[2-[2-[[2-[[6-amino-2-[[4-amino-2-[[2-[(2-aminoacetyl)amino]-3-hydroxypropanoyl]amino]-4-oxobutanoyl]amino]hexanoyl]amino]acetyl]amino]propanoylamino]-3-methylpentanoyl]amino]-3-methylpentanoyl]amino]acetyl]amino]-4-methylpentanoyl]amino]-4-methylsulfanylbutanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product is not stable in solution, please use freshly prepared working solution for optimal results.(2). Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The amino acid sequence of Aβ(25-35) peptide is NH2-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH, where the first Gly represents amino acid 25, and the second Gly Represents amino acid 25. The last Met stands for amino acid 35. Amyloid β-peptide (25-35) was also investigated in gel form for the first time. Comparative investigations were also undertaken using vibration absorption and ECD. The structural preferences of Aβ(25-35) peptide films were also examined using vibrational absorption and VCD spectroscopy [1]. Amyloid β-peptide (25-35) can trigger apoptosis in isolated brain mitochondria and methionine-35 redox state, playing a vital role in generating programmed cell death pathways and toxic events [2]. Recommendations for Beta-Amyloid Aggregation (This is our suggested approach; it should be adjusted to suit your particular need as it simply offers guidelines). 1. Solid Aβ peptide should be dissolved in cold hexafluoro-2-propanol (HFIP). To achieve monomerization and randomization of the structure, incubate the peptides for a minimum of one hour at room temperature. 2. To get rid of HFIP, evaporate, and then store the resultant peptide in a film at -20 or -80°C. 3. The resultant membrane should be dissolved in 5 mM of anhydrous DMSO, vortexed, and diluted with buffer (serum- and phenol-red-free media) to the proper concentration. 4. After that, let the solution age for 48 hours at 4–8°C. After centrifuging the samples for 10 minutes at 4–8°C at 14,000 g, the supernatant contained soluble oligomers. For the studies, the supernatant was diluted 10-200 times. Different approaches are used based on the final application.
ln Vivo	Alzheimer's disease models can be created using animal models with β-Amyloid (25–35).
References	[1]. Solution structure of amyloid beta-peptide (25-35) in different media. J Med Chem. 2004 Aug 12;47(17):4231-8. [2]. Abeta(31-35) and Abeta(25-35) fragments of amyloid beta-protein induce cellular death throughapoptotic signals: Role of the redox state of methionine-35. FEBS Lett. 2005 May 23;579(13):2913-8. [3]. Cognitive improvement of compound danshen in an Aβ25-35 peptide-induced rat model of Alzheimer's disease. BMC Complement Altern Med. 2015 Oct 23;15:382. [4]. Potent anti-angiogenic motifs within the Alzheimer beta-amyloid peptide. Amyloid. 2008;15(1):5-19. [5]. Ropren® is a polyprenol preparation from coniferous plants that ameliorates cognitive deficiency in a rat model of beta-amyloid peptide-(25–35)-induced amnesia. Phytomedicine. 2012 Mar 15;19(5):451-6.

Solubility Data

Solubility (In Vitro)

H2O : ≥ 3.33 mg/mL (~3.14 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (2.36 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.9432 mL	4.7158 mL	9.4316 mL
	5 mM	0.1886 mL	0.9432 mL	1.8863 mL
	10 mM	0.0943 mL	0.4716 mL	0.9432 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.