Alisol F 24-acetate is a triterpene compound extracted from the rhizome of Alisma orientalis. Alisol F 24-acetate inhibits the secretion of HBV (hepatitis B virus) surface antigens HBsAg and HBeAg with IC50s of 7.7 µM and 5.1 µM, respectively. Alisol F 24-acetate has pro-apoptotic activity and may be used in cancer research.

Physicochemical Properties

Molecular Formula	C32H50O6
Molecular Weight	530.735810756683
Exact Mass	530.36
CAS #	443683-76-9
PubChem CID	76310823
Appearance	Typically exists as solid at room temperature
Density	1.2±0.1 g/cm3
Boiling Point	634.3±55.0 °C at 760 mmHg
Flash Point	195.8±25.0 °C
Vapour Pressure	0.0±4.2 mmHg at 25°C
Index of Refraction	1.552
LogP	4.54
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	4
Heavy Atom Count	38
Complexity	1060
Defined Atom Stereocenter Count	10
SMILES	CC(O[C@H]([C@@H]1C[C@@H](C)C2=C3C[C@H](O)C4[C@]5(CCC(=O)C(C)(C)C5CC[C@]4(C)[C@@]3(C)C[C@@H]2O1)C)C(O)(C)C)=O
InChi Key	KFWYQAKZMXFEFB-XKFNBYHKSA-N
InChi Code	InChI=1S/C32H50O6/c1-17-14-21(27(29(5,6)36)37-18(2)33)38-22-16-32(9)19(25(17)22)15-20(34)26-30(7)12-11-24(35)28(3,4)23(30)10-13-31(26,32)8/h17,20-23,26-27,34,36H,10-16H2,1-9H3/t17-,20+,21+,22+,23+,26+,27-,30+,31+,32+/m1/s1
Chemical Name	[(1R)-2-hydroxy-1-[(1S,2R,4S,6S,8R,12S,13S,14S,19R)-12-hydroxy-1,2,8,14,18,18-hexamethyl-17-oxo-5-oxapentacyclo[11.8.0.02,10.04,9.014,19]henicos-9-en-6-yl]-2-methylpropyl] acetate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In MCF-7/DOX cells, Alisol F 24-acetate (5, 10 and 20 µM; 24 h) increases the chemosensitivity to doxorubicin (HY-15142A) in a dose-dependent manner [2]. The accumulation of doxorubicin in MCF-7/DOX cells was enhanced in a dose-dependent manner by Alisol F 24-acetate at 5, 10, and 20 µM. In Caco-2 cell monolayers, Alisol F 24-acetate (10 µM) improves digoxin (HY-B1049) absorption (AP-BL) and reduces digoxin secretion (BL-AP) [2].
Cell Assay	Cell Viability Assay[2] Cell Types: Caco-2 cells and MCF-7/DOX cells. Tested Concentrations: 1, 2, 5, 10, 20, 50 and 100 µM. Incubation Duration: 24 h. Experimental Results: Dramatically inhibited the cell viability (100 µM). Apoptosis Analysis[2] Cell Types: MCF-7/DOX cells. Tested Concentrations: 5, 10 and 20 µM. Incubation Duration: 0.5, 1, 2, 3 and 4 h. Experimental Results: Promoted the doxorubicin-induced apoptosis with time and dose dependent manner.
References	[1]. A new triterpene and anti-hepatitis B virus active compounds from Alisma orientalis. Planta Med. 2006 Aug;72(10):951-4. [2]. Alisol F 24 Acetate Enhances Chemosensitivity and Apoptosis of MCF-7/DOX Cells by Inhibiting P-Glycoprotein-Mediated Drug Efflux. Molecules. 2016 Feb 4;21(2):183.

Solubility Data

Solubility (In Vitro)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

Solubility (In Vivo)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.8842 mL	9.4208 mL	18.8416 mL
	5 mM	0.3768 mL	1.8842 mL	3.7683 mL
	10 mM	0.1884 mL	0.9421 mL	1.8842 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.