Physicochemical Properties
| Molecular Formula | C27H28CL4N6 |
| Molecular Weight | 578.363 |
| Exact Mass | 576.112 |
| CAS # | 69235-50-3 |
| Related CAS # | Acriflavine;8048-52-0 |
| PubChem CID | 443101 |
| Appearance | Brown to reddish brown solid powder |
| Hydrogen Bond Donor Count | 4 |
| Hydrogen Bond Acceptor Count | 6 |
| Rotatable Bond Count | 0 |
| Heavy Atom Count | 34 |
| Complexity | 486 |
| Defined Atom Stereocenter Count | 0 |
| InChi Key | PEJLNXHANOHNSU-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C14H13N3.C13H11N3.ClH/c1-17-13-7-11(15)4-2-9(13)6-10-3-5-12(16)8-14(10)17;14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;/h2-8H,1H3,(H3,15,16);1-7H,14-15H2;1H |
| Chemical Name | acridine-3,6-diamine;10-methylacridin-10-ium-3,6-diamine;chloride |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| References |
[1]. Interaction of acriflavine with DNA and RNA. J Mol Biol. 1969 Mar 28;40(3):491-5. 2.0.CO;2">[2]. Effects of Calcium Content and Humic Substances on the Toxicity of Acriflavine to Juvenile Zebrafish Danio rerio. Journal of Aquatic Animal Health, 14:1, 35-38. [3]. HIF-1 Dimerization Inhibitor Acriflavine Enhances Antitumor Activity of Sunitinib in Breast Cancer Model. Oncol Res. 2015; 22(3): 139-145. [4]. Acriflavine, a Potent Inhibitor of HIF-1α, Disturbs Glucose Metabolism and Suppresses ATF4-Protective Pathways in Melanoma under Non-Hypoxic Conditions. Cancers (Basel). 2021 Jan; 13(1): 102. [5]. A putative transcription regulator involved in the virulence attenuation of an acriflavine-resistant vaccine strain of Erysipelothrix rhusiopathiae, the causative agent of swine erysipelas. Vet Microbiol. 2019 Dec;239:108488. [6]. Acriflavine, a clinically aproved drug, inhibits SARS-CoV-2 and other betacoronaviruses. bioRxiv 2021.03.20.436259. |
| Additional Infomation |
3,6-Diamino-10-methylacridinium chloride mixt. with 3,6-acridinediamine. Fluorescent dye used as a local antiseptic and also as a biological stain. It intercalates into nucleic acids thereby inhibiting bacterial and viral replication. See also: Acriflavine (annotation moved to). |
Solubility Data
| Solubility (In Vitro) | DMSO : ~25 mg/mL (~46.13 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (4.61 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (4.61 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7290 mL | 8.6451 mL | 17.2903 mL | |
| 5 mM | 0.3458 mL | 1.7290 mL | 3.4581 mL | |
| 10 mM | 0.1729 mL | 0.8645 mL | 1.7290 mL |