Acetylleucine (N-Acetyl-DL-leucine), an orally biologically active compound, may be utilized in study/research of acute vestibular vertigo, cerebellar ataxia, and nystagmus.

Physicochemical Properties

Molecular Formula	C8H15NO3
Molecular Weight	173.2096
Exact Mass	173.105
CAS #	99-15-0
PubChem CID	1995
Appearance	White to off-white solid powder
Density	1.1±0.1 g/cm3
Boiling Point	369.6±25.0 °C at 760 mmHg
Melting Point	160ºC
Flash Point	177.4±23.2 °C
Vapour Pressure	0.0±1.8 mmHg at 25°C
Index of Refraction	1.458
LogP	0.34
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	4
Heavy Atom Count	12
Complexity	177
Defined Atom Stereocenter Count	0
SMILES	O([H])C(C([H])(C([H])([H])C([H])(C([H])([H])[H])C([H])([H])[H])N([H])C(C([H])([H])[H])=O)=O
InChi Key	WXNXCEHXYPACJF-UHFFFAOYSA-N
InChi Code	InChI=1S/C8H15NO3/c1-5(2)4-7(8(11)12)9-6(3)10/h5,7H,4H2,1-3H3,(H,9,10)(H,11,12)
Chemical Name	2-acetamido-4-methylpentanoic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Additional Infomation

N-acetyl-leucine is a leucine derivative and a N-acetyl-amino acid. Used for treating vestibular-related imbalance and vertigo.

Solubility Data

Solubility (In Vitro)

DMSO : ~120 mg/mL (~692.80 mM) H2O : ~6.67 mg/mL (~38.51 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 3 mg/mL (17.32 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 30.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 3 mg/mL (17.32 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 30.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 3 mg/mL (17.32 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 30.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 4: 4 mg/mL (23.09 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C).  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.7733 mL	28.8667 mL	57.7334 mL
	5 mM	1.1547 mL	5.7733 mL	11.5467 mL
	10 mM	0.5773 mL	2.8867 mL	5.7733 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.