Acetyl-6-formylpterin (Ac-6-FP) is a hapten that covalently binds to MR1 via residue Lys43 of the protein, leading to upregulation of the protein. Acetyl-6-formylpterin (Ac-6-FP) can be used to study inflammation.

Physicochemical Properties

Molecular Formula	C9H7N5O3
Molecular Weight	233.18
Exact Mass	233.055
CAS #	29769-49-1
PubChem CID	135447887
Appearance	White to yellow solid powder
Density	1.72g/cm3
Index of Refraction	1.778
LogP	-1.4
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	2
Heavy Atom Count	17
Complexity	394
Defined Atom Stereocenter Count	0
InChi Key	DDBCPKAHJKOGKK-UHFFFAOYSA-N
InChi Code	InChI=1S/C9H7N5O3/c1-4(16)11-9-13-7-6(8(17)14-9)12-5(3-15)2-10-7/h2-3H,1H3,(H2,10,11,13,14,16,17)
Chemical Name	N-(6-formyl-4-oxo-3H-pteridin-2-yl)acetamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1].Properties and reactivity of the folic acid and folate photoproduct 6-formylpterin. Free Radic Biol Med. 2021 Aug 1;171:1-10.
Additional Infomation	N(2)-acetyl-6-formylpterin is pterin acetylated on N-2 and carrying a formyl group at position 6.

Solubility Data

Solubility (In Vitro)

DMSO :~100 mg/mL (~428.85 mM; with sonication) DMF :~50 mg/mL (~214.43 mM; with heating and sonication.)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (10.72 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared,you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 900 μL of 20% SBE-β-CD saline solution and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.72 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared,you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 900 μL corn oil and mix well.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.2885 mL	21.4427 mL	42.8853 mL
	5 mM	0.8577 mL	4.2885 mL	8.5771 mL
	10 mM	0.4289 mL	2.1443 mL	4.2885 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.