Physicochemical Properties
| Molecular Formula | C40H38F6N8O13 |
| Molecular Weight | 952.77 |
| Related CAS # | Ac-WEHD-AFC;210344-99-3 |
| Appearance | Off-white to light yellow solid powder |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| Targets | Caspase-1 |
| ln Vitro | Rules(This is the suggested procedure. This protocol should be customized to meet your unique requirements, as it just offers guidelines. Assay for Caspase-1 Fluorogenic Activity[1]: 1. Follow your standard protocol for cell culture. 2. After 24 hours, cells that have been transfected transiently with caspase-1 in conjunction with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP are harvested. 3. For cleavage experiments, cells were lysed in CHEGG buffer and then sonicated for 15 seconds at 60% amplitude. 4. 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC TFA was added to the lysates during incubation. 5. The release of free AFC was measured as arbitrary fluorescence units per minute and tracked continuously for one hour (excitation at 380 nm and emission at 460 nm) at one-minute intervals. |
| References |
[1]. Cellular inhibitors of apoptosis proteins cIAP1 and cIAP2 are required for efficient caspase-1 activation by the inflammasome. Immunity. 2011 Dec 23;35(6):897-907. |
Solubility Data
| Solubility (In Vitro) |
DMSO :~100 mg/mL (~104.96 mM) H2O :~50 mg/mL (~52.48 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.62 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.62 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (2.62 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0496 mL | 5.2479 mL | 10.4957 mL | |
| 5 mM | 0.2099 mL | 1.0496 mL | 2.0991 mL | |
| 10 mM | 0.1050 mL | 0.5248 mL | 1.0496 mL |