AZD4694 Precursor (AZ13040214) is the precursor of [18F] AZD4694 and is used to synthesize [18F] AZD4694. AZD4694 Precursor is an amyloid-beta imaging ligand with high affinity for amyloid-beta plaques.

Physicochemical Properties

Molecular Formula	C22H25N3O7
Molecular Weight	443.45
Exact Mass	443.169
CAS #	1211333-20-8
PubChem CID	46183972
Appearance	Light green to yellow green solid powder
Density	1.3±0.1 g/cm3
Boiling Point	593.4±50.0 °C at 760 mmHg
Flash Point	312.7±30.1 °C
Vapour Pressure	0.0±1.7 mmHg at 25°C
Index of Refraction	1.594
LogP	3.65
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	8
Heavy Atom Count	32
Complexity	650
Defined Atom Stereocenter Count	0
InChi Key	YDLUIXGYKWYMMC-UHFFFAOYSA-N
InChi Code	InChI=1S/C22H25N3O7/c1-6-29-13-30-15-7-9-17-14(11-15)12-18(31-17)16-8-10-19(23-20(16)25(27)28)24(5)21(26)32-22(2,3)4/h7-12H,6,13H2,1-5H3
Chemical Name	tert-butyl N-[5-[5-(ethoxymethoxy)-1-benzofuran-2-yl]-6-nitropyridin-2-yl]-N-methylcarbamate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The amyloid-β imaging ligand AZD4694 Precursor has a strong affinity for amyloid-β plaques. When compared to other fluorinated amyloid-PET tracers, AZD4694 Precursor exhibits lesser white matter binding, making it a high affinity (Kd= 2.3 nM) radioligand for imaging amyloid-β plaques that facilitates easier visual reads[1].
References	[1]. Determining Amyloid-β Positivity Using 18F-AZD4694 PET Imaging. J Nucl Med. 2021;62(2):247-252.

Solubility Data

Solubility (In Vitro)

DMSO: 50 mg/mL (112.75 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (5.64 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.2550 mL	11.2752 mL	22.5505 mL
	5 mM	0.4510 mL	2.2550 mL	4.5101 mL
	10 mM	0.2255 mL	1.1275 mL	2.2550 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.