AVJ16 is a cancer cell migration inhibitor that targets the insulin-like growth factor 2 mRNA-binding protein IGF2BP1 and can inhibit the migration of H1299 cells that highly express endogenous IGF2BP1. AVJ16 interferes with IGF2BP1 binding to target mRNA to regulate gene expression and translation.

Physicochemical Properties

Molecular Formula	C28H27N3O4
Molecular Weight	469.531687021255
Exact Mass	469.2
CAS #	2775241-92-2
PubChem CID	163196422
Appearance	Off-white to light yellow solid powder
LogP	3.6
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	5
Heavy Atom Count	35
Complexity	721
Defined Atom Stereocenter Count	0
InChi Key	SBMDRXUGWAAAMV-UHFFFAOYSA-N
InChi Code	InChI=1S/C28H27N3O4/c32-18-24-15-22-3-1-2-4-25(22)31(24)17-20-5-7-21(8-6-20)28(33)30-13-11-29(12-14-30)23-9-10-26-27(16-23)35-19-34-26/h1-10,15-16,32H,11-14,17-19H2
Chemical Name	[4-(1,3-benzodioxol-5-yl)piperazin-1-yl]-[4-[[2-(hydroxymethyl)indol-1-yl]methyl]phenyl]methanone
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Insulin-like growth factor 2 mRNA binding protein (IGF2BP1)[1]
References	[1]. Development of a Novel IGF2BP1 Inhibitor as Metastasis-Specific Therapeutic Agent. European Journal of Cancer, 2022, 174: S104-S105.

Solubility Data

Solubility (In Vitro)

DMSO: 250 mg/mL (532.45 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (4.43 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.43 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1298 mL	10.6489 mL	21.2979 mL
	5 mM	0.4260 mL	2.1298 mL	4.2596 mL
	10 mM	0.2130 mL	1.0649 mL	2.1298 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.