ATWLPPRAANLLMAAS is a chimeric peptide with anti-angiogenic and potent anti-tumor effects. ATWLPPRAANLLMAAS inhibits proliferation, viability, migration and invasion of human hepatocellular carcinoma cells and induces apoptosis.

Physicochemical Properties

Molecular Formula	C76H123N21O20S
Molecular Weight	1682.98
CAS #	1228447-26-4
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	ATWLPPRAANLLMAAS (V3) (25-200 μM; 12-24 h) inhibits the proliferation, viability, migration and invasion of human HCC (SMMC-7721, Huh-7) cells by inhibiting the Ras/Raf/MEK/ERK signaling pathway, however, no significant effect was observed in HL-7702 cells[2]. ATWLPPRAANLLMAAS (200 μM; 24 h) increased apoptosis and decreased the protein levels of H-RAS, phosphorylated (p)-RAF, p-MEK and p-extracellular signal-regulated protein kinase (ERK) in human HCC (SMMC-7721, Huh-7) cells[2]. Cell Migration Assay [2] Cell Line: HL-7702, SMMC-7721, Huh-7 Concentration: 25, 50, 100, 200 µM Incubation Time: 12h, 24 h Result: Inhibited the migration of SMMC-7721 and Huh-7 cells in a dose-dependent manner, but had no effect on HL-7702 cells.
ln Vivo	ATWLPPRAANLLMAAS (160-480 μg/kg; sc; once a day for 7 consecutive days) can significantly inhibit the growth of mouse sarcoma S180 and H22 liver cancer xenografts in a dose-dependent manner [1]. ATWLPPRAANLLMAAS (200 μg/kg; sc; once a day for 4 weeks) inhibits the growth of human HCC xenografts by downregulating angiogenesis and upregulating apoptosis [2].
Cell Assay	Apoptosis Analysis[2] Cell Types: SMMC-7721, Huh-7 Tested Tested Concentrations: 200 μM Incubation Duration: 24 h Experimental Results: Remarkably increased the apoptotic index, protein expressions of cleaved caspase-3, 8, 9, cleaved PARP, as well as Bax/Bcl-2 and Bad/Bcl-xl ratios.
References	[1]. Anti-tumor effects of a novel chimeric peptide on S180 and H22 xenografts bearing nude miceJ. Peptides, 2010, 31(5): 850-864. [2]. Peptide V3 inhibits the growth of human hepatocellular carcinoma by inhibiting the Ras/Raf/MEK/ERK signaling pathwayJ. Journal of Cancer, 2019, 10(7): 1693.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.5942 mL	2.9709 mL	5.9418 mL
	5 mM	0.1188 mL	0.5942 mL	1.1884 mL
	10 mM	0.0594 mL	0.2971 mL	0.5942 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.