ATF4-IN-1 (Compound 21) is an ATF4 inhibitor and an activator of eIF2B. ATF4-IN-1 can be used in neurodegenerative disease research.

Physicochemical Properties

Molecular Formula	C24H21CL2N3O4
Molecular Weight	486.35
CAS #	2991057-76-0
Appearance	White to off-white solid powder
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	ATF4-IN-1 inhibits the expression of ATF4 in HEK-293T cells in a dose-dependent manner with an IC50 of 32.43 nM[1]. ATF4-IN-1 (0-1000 nM, 3 h) inhibits the protein expression of ATF4 in HEK-293T cells[1]. ATF4-IN-1 (200 nM, 3 h) inhibits the transcription of ATF4 mRNA in HeLa cells[1]. ATF4-IN-1 activates eIF2B with an EC50 of 5.844 nM in HEK-293T cells[1]. ATF4-IN-1 inhibits the proliferation of HEK-293T cells with an IC50 of 96 μM.
Cell Assay	Western Blot Analysis[1] Cell Types: HEK-293 T Tested Tested Concentrations: 0, 1, 10, 100, 500, 1000 nM Incubation Duration: 3 h Experimental Results: Inhibited ATF4 expression. Real Time qPCR[1] Cell Types: HeLa Tested Tested Concentrations: 200 nM Incubation Duration: 3 h Experimental Results: Inhibited mRNA transcription of ATF4.
References	[1]. Design, Synthesis, and Biological Evaluation of Eukaryotic Initiation Factor 2B (eIF2B) Activators. ChemMedChem. 2024 Mar 1:e202300716.

Solubility Data

Solubility (In Vitro)

DMSO : ~8.33 mg/mL (~17.13 mM; with ultrasonication (<60°C))

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0561 mL	10.2807 mL	20.5613 mL
	5 mM	0.4112 mL	2.0561 mL	4.1123 mL
	10 mM	0.2056 mL	1.0281 mL	2.0561 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.