ATF3 inducer 1 is a potent ATF3 inducer. ATF3 inducer 1 increases the expression of ATF3 protein and ATF3 mRNA. ATF3 inducer displays anti-MetS activity in mice.

Physicochemical Properties

Molecular Formula	C12H10N2O3
Molecular Weight	230.22
Appearance	White to light yellow solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In 3T3-L1 cells, ATF3 inducer 1 (compound 16c) (50 μM; 8 days) stimulates the expression of ATF3 mRNA and protein[1].
ln Vivo	ATF3 inducer 1 (40 mg/kg; intraperitoneally; three times per week for ten weeks) has anti-metabolic syndrome (MetS) action in mice[1].
Cell Assay	Western Blot Analysis[1] Cell Types: 3T3-L1 cells Tested Concentrations: 50 μM Incubation Duration: 8 days Experimental Results: Increased the ATF3 protein and ATF3 mRNA expression, demonstrated no lipid accumulation.
Animal Protocol	Animal/Disease Models: Eightweeks old C57BL/6 male mice[ 1] Doses: 40 mg/kg Route of Administration: Ip; three times a week for 10-weeks Experimental Results: diminished the bodyweight and the size of epididymal white adipose tissue (eWAT) adipocytes was Dramatically diminished.
References	[1]. Exploration and biological evaluation of 7-methoxy-3-methyl-1H-chromeno[4,3-c]pyrazol-4-one as an activating transcription factor 3 inducer for managing metabolic syndrome. Eur J Med Chem. 2022 Nov 25;246:114951.

Solubility Data

Solubility (In Vitro)

DMSO :~65 mg/mL (~282.34 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (9.03 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (9.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.3437 mL	21.7184 mL	43.4367 mL
	5 mM	0.8687 mL	4.3437 mL	8.6873 mL
	10 mM	0.4344 mL	2.1718 mL	4.3437 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.