ARC 239 diHCl is a selective α2B/2C adrenergic receptor blocker (antagonist) (pKds for α2A, α2B, and α2C receptors are 5.95, 7.41, and 7.56, respectively). ARC 239 diHCl binds to CHO cell membranes expressing recombinant human a2A-, a2B-, or a2C-adrenergic receptor subtypes with pKi of 5.6, 8.4, and 7.08, respectively.

Physicochemical Properties

Molecular Formula	C24H31CL2N3O3
Molecular Weight	480.43
Exact Mass	479.174
Elemental Analysis	C, 60.00; H, 6.50; Cl, 14.76; N, 8.75; O, 9.99
CAS #	55974-42-0
Related CAS #	ARC 239; 67339-62-2
PubChem CID	10345075
Appearance	White to off-white solid powder
LogP	4.322
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	5
Heavy Atom Count	32
Complexity	629
Defined Atom Stereocenter Count	0
SMILES	Cl.Cl.O=C1C(C)(C)C2C=CC=CC=2C(N1CCN1CCN(C2C=CC=CC=2OC)CC1)=O
InChi Key	SAJKHRHHDGSJEZ-UHFFFAOYSA-N
InChi Code	InChI=1S/C24H29N3O3.2ClH/c1-24(2)19-9-5-4-8-18(19)22(28)27(23(24)29)17-14-25-12-15-26(16-13-25)20-10-6-7-11-21(20)30-3;;/h4-11H,12-17H2,1-3H3;2*1H
Chemical Name	2-[2-[4-(2-methoxyphenyl)piperazin-1-yl]ethyl]-4,4-dimethylisoquinoline-1,3-dione;dihydrochloride
Synonyms	ARC239 2HCl; ARC 239 2HCl; ARC-239 2HCl; ARC239 dihydrochloride; ARC-239 dihydrochloride; ARC 239 dihydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	5-HT1A receptor; Alpha-2B adrenergic receptor
References	[1]. Pharmacological characterization of alpha 2-adrenoceptor-mediated responses in pig nasal mucosa. Auton Autacoid Pharmacol. 2003 Aug;23(4):208-19.

Solubility Data

Solubility (In Vitro)

DMSO: ~125 mg/mL (~260.2 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (4.33 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.33 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.08 mg/mL (4.33 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0815 mL	10.4073 mL	20.8147 mL
	5 mM	0.4163 mL	2.0815 mL	4.1629 mL
	10 mM	0.2081 mL	1.0407 mL	2.0815 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.