7-Amino-4-(trifluoromethyl)coumarin (Coumarin 151) is a fluorescent label that may be utilized to sensitively detect proteases. Its excitation and emission wavelengths are 400 and 500 nm respectively.

Physicochemical Properties

Molecular Formula	C10H6F3NO2
Molecular Weight	229.1554
Exact Mass	229.035
CAS #	53518-15-3
PubChem CID	100641
Appearance	Light yellow to yellow solid powder
Density	1.5±0.1 g/cm3
Boiling Point	314.6±42.0 °C at 760 mmHg
Melting Point	221-222 °C(lit.)
Flash Point	144.1±27.9 °C
Vapour Pressure	0.0±0.7 mmHg at 25°C
Index of Refraction	1.562
LogP	1.64
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	0
Heavy Atom Count	16
Complexity	337
Defined Atom Stereocenter Count	0
InChi Key	JBNOVHJXQSHGRL-UHFFFAOYSA-N
InChi Code	InChI=1S/C10H6F3NO2/c11-10(12,13)7-4-9(15)16-8-3-5(14)1-2-6(7)8/h1-4H,14H2
Chemical Name	7-amino-4-(trifluoromethyl)chromen-2-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The fluorescent compound N-acetyl-asp-glu-val-asp-7-amino-4-(trifluoromethyl)coumarin (Ac-DEVD-AFC) was used to test caspase activators. The substrate penetrates the cell quickly and is effectively broken down at aspartic acid residues by certain caspases, resulting in the fluorescent molecule 7-amino-4-(trifluoromethyl)coumarin (AFC). The liberated 7-Amino-4-(trifluoromethyl)coumarin was isolated on HPLC and identified by fluorescence upon cell rupture. The intracellular caspase responsible for the emergence of intracellular 7-Amino-4-(trifluoromethyl)coumarin is identified [1] as a burst of the pancaspase benzyloxycarbonyl-val-ala-asp-fluoromethylketone. The synthesis of γ-glutamyl-7-amino-4-(trifluoromethyl)coumarin helped determine the fluorescence of γ-glutamyl transpeptidase by assisting the substrate reaction product 7-amino-4-(trifluoromethyl). With excitation and emission wavelengths of 400 and 490 nm, respectively, and a linear correlation with phosphors in the concentration range of 10-300 pmol/3 mL, coumarin responds linearly with phosphors at neutral pH. blend[2].
References	[1]. Caspase activation by anticancer drugs: the caspase storm. Mol Pharm. 2007 Jul-Aug;4(4):583-95. [2]. A continuous fluorometric assay for gamma-glutamyltranspeptidase. Anal Biochem. 1996 Jan 1;233(1):71-5.
Additional Infomation	7-amino-4-(trifluoromethyl)coumarin is a member of 7-aminocoumarins. It has a role as a fluorochrome.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~436.38 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (10.91 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.3638 mL	21.8188 mL	43.6376 mL
	5 mM	0.8728 mL	4.3638 mL	8.7275 mL
	10 mM	0.4364 mL	2.1819 mL	4.3638 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.