AEG-3482 is a novel and potent antiapoptotic agent that inhibits c-Jun kinase (JNK) activity by inducing the expression of heat shock protein 70 (HSP70).
Physicochemical Properties
| Molecular Formula | C10H8N4O2S2 |
| Molecular Weight | 280.32612 |
| Exact Mass | 280.008 |
| Elemental Analysis | C, 42.85; H, 2.88; N, 19.99; O, 11.41; S, 22.87 |
| CAS # | 63735-71-7 |
| Related CAS # | 63735-71-7 |
| PubChem CID | 698112 |
| Appearance | White to light yellow solid powder |
| Density | 1.8±0.1 g/cm3 |
| Index of Refraction | 1.835 |
| LogP | 1.32 |
| Hydrogen Bond Donor Count | 1 |
| Hydrogen Bond Acceptor Count | 6 |
| Rotatable Bond Count | 2 |
| Heavy Atom Count | 18 |
| Complexity | 405 |
| Defined Atom Stereocenter Count | 0 |
| SMILES | C1=CC=C(C=C1)C2=CN3C(=N2)SC(=N3)S(=O)(=O)N |
| InChi Key | MQUYTXDAVCOCMX-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C10H8N4O2S2/c11-18(15,16)10-13-14-6-8(12-9(14)17-10)7-4-2-1-3-5-7/h1-6H,(H2,11,15,16) |
| Chemical Name | 6-phenylimidazo[2,1-b][1,3,4]thiadiazole-2-sulfonamide |
| Synonyms | AEG-3482; AEG 3482; AEG3482 |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| Targets | JNK |
| ln Vitro |
AEG3482 (0.3-30 μM; 2 d) inhibits nerve growth factor (NGF) withdrawal-induced death in SCG neurons, with an EC50 of ∼20 μM[1]. AEG3482 (1-80 μM; 40 h) inhibits PC12 cells' p75NTR- and NRAGE-mediated apoptosis in a dose-dependent manner[1]. AEG3482 (10-40 μM; 30 h) inhibits p75NTR- and NRAGE-mediated JNK activation in PC12 cells[1]. |
| References |
[1]. AEG3482 is an antiapoptotic compound that inhibits Jun kinase activity and cell death through induced expression of heat shock protein 70. Chem Biol. 2006 Feb;13(2):213-23. |
| Additional Infomation | 6-phenyl-2-imidazo[2,1-b][1,3,4]thiadiazolesulfonamide is a member of imidazoles. |
Solubility Data
| Solubility (In Vitro) | DMSO: ~250 mg/mL (~891.8 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.42 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.42 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5672 mL | 17.8361 mL | 35.6722 mL | |
| 5 mM | 0.7134 mL | 3.5672 mL | 7.1344 mL | |
| 10 mM | 0.3567 mL | 1.7836 mL | 3.5672 mL |