ADA is a biological buffer. ADA can be used as a chelating agent.

Physicochemical Properties

Molecular Formula	C6H10N2O5
Molecular Weight	190.1540
Exact Mass	190.058
CAS #	26239-55-4
PubChem CID	117765
Appearance	White to off-white solid powder
Density	1.5±0.1 g/cm3
Boiling Point	524.9±45.0 °C at 760 mmHg
Melting Point	219 °C (dec.)(lit.)
Flash Point	271.3±28.7 °C
Vapour Pressure	0.0±3.0 mmHg at 25°C
Index of Refraction	1.558
LogP	-3.9
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	6
Heavy Atom Count	13
Complexity	211
Defined Atom Stereocenter Count	0
InChi Key	QZTKDVCDBIDYMD-UHFFFAOYSA-N
InChi Code	InChI=1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13)
Chemical Name	2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. T Minami, et al. Identification of metallothionein isoforms with capillary zone electrophoresis using a polyacrylamide-coated tube. J Chromatogr B Biomed Appl. 1996 Oct 25;685(2):353-9. [2]. Birte M Gerken, et al. Tweezing-adsorptive bubble separation. Analytical method for the selective and high enrichment of metalloenzymes. Anal Chem. 2005 Oct 1;77(19):6113-7.
Additional Infomation	2,2'-[(2-amino-2-oxoethyl)imino]diacetic acid is a tricarboxylic acid amide that is a Good's buffer substance, pKa = 6.6 at 20 ℃. It is a dicarboxylic acid, a tricarboxylic acid amide and an ADA. It is functionally related to a nitrilotriacetic acid. It is a conjugate acid of a 2,2'-[(2-amino-2-oxoethyl)imino]diacetate(1-).

Solubility Data

Solubility (In Vitro)	H2O: 5 mg/mL (26.30 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 10 mg/mL (52.59 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.2590 mL	26.2950 mL	52.5901 mL
	5 mM	1.0518 mL	5.2590 mL	10.5180 mL
	10 mM	0.5259 mL	2.6295 mL	5.2590 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.