ACTH (1-17) TFA is an adrenocorticotropic hormone analogue and a potent human melanocortin 1 (MC1) receptor agonist/activator with a Ki of 0.21 nM.

Physicochemical Properties

Molecular Formula	C95H145N29O23S.C2HF3O2
Molecular Weight	2207.43
Related CAS #	ACTH (1-17);7266-47-9
Appearance	Solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	MC1R
ln Vitro	ACTH.(1–17) Strong agonists of hMC1R include TFA. With a Ki value of 0.21±0.03 nM, the affinity of ACTH(1-17) for hMC1R is high, marginally higher than that of alpha-MSH at 0.13±0.005 nM[1]. Acth(1-17) stimulates growth hormone production in rat pituitary cultures even at micromolar doses [2], albeit not significantly.
ln Vivo	Two hours after the daily dark span begins, nocturnal animals become active, and inhibition of DNA tagging is observed when ACTH (1-17) is delivered. Larger doses in particular were linked to a 24-hour reduction of DNA labeling when given during this circadian phase. The inhibitory impact was significantly reduced when the same amount was administered four hours earlier [3].
References	[1]. ACTH1-17 is a more potent agonist at the human MC1 receptor than alpha-MSH. Cell Mol Biol (Noisy-le-grand). 1999 Nov;45(7):1029-34. [2]. The effects of ACTH (1-17) on GH secretion in vitro. Horm Metab Res. 1987 Aug;19(8):361-3. [3]. Effect of an adrenocorticotropin analogue, ACTH (1-17), on DNA synthesis in murine metaphyseal bone. Biochem Pharmacol. 1985 Apr 15;34(8):1191-6.

Solubility Data

Solubility (In Vitro)	H2O :~100 mg/mL (~45.30 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (45.30 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.4530 mL	2.2651 mL	4.5302 mL
	5 mM	0.0906 mL	0.4530 mL	0.9060 mL
	10 mM	0.0453 mL	0.2265 mL	0.4530 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.