AAK1/HDACs-IN-1 (Compound 12) is a dual inhibitor of AAK1 and HDAC, inhibiting AAK1, HDAC1, and HDAC6 with IC50 of 15.9, 148.6, and 5.2 nM, respectively. AAK1/HDACs-IN-1 inhibits SARS-CoV-2 infection, endocytosis of the ACE2-SARS-CoV-2 complex, and AP2M1-ACE2 interaction.

Physicochemical Properties

Molecular Formula	C21H14N4O2
Molecular Weight	354.36
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	HDAC6 5.2 nM (IC50); AAK1 15.9 nM (IC50); HDAC1 148.6 nM (IC50)
References	[1]. Design, synthesis, and biological evaluation of novel AAK1/HDACs dual inhibitors against SARS-CoV-2 entry. Bioorg Chem. 2024 Dec;153:107973.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.8220 mL	14.1099 mL	28.2199 mL
	5 mM	0.5644 mL	2.8220 mL	5.6440 mL
	10 mM	0.2822 mL	1.4110 mL	2.8220 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.