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A 21960 is able to inhibit the synapse formation between resolvase and two directly repeated res sites, and subsequently block the site-specific recombination reaction. The Tn3-encoded resolvase protein promotes a site-specific recombination reaction between two directly repeated copies of the recombination site res. A21960 does not affect resolvase binding and bending of the DNA but inhibit synapse formation between resolvase and two directly repeated res sites.
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Physicochemical Properties
| Molecular Formula |
C6H9CLO3
|
| Molecular Weight |
164.5869
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| Exact Mass |
164.024
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| CAS # |
609-15-4
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| Related CAS # |
|
| PubChem CID |
11858
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| Appearance |
Colorless to light yellow liquid（Density：1.172 g/cm³）
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
225.5±0.0 °C at 760 mmHg
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| Melting Point |
-80 °C
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| Flash Point |
50.0±0.0 °C
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| Vapour Pressure |
0.1±0.4 mmHg at 25°C
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| Index of Refraction |
1.434
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| LogP |
1.66
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
10
|
| Complexity |
144
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| Defined Atom Stereocenter Count |
0
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| SMILES |
ClC([H])(C(C([H])([H])[H])=O)C(=O)OC([H])([H])C([H])([H])[H]
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| InChi Key |
RDULEYWUGKOCMR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H9ClO3/c1-3-10-6(9)5(7)4(2)8/h5H,3H2,1-2H3
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| Chemical Name |
ethyl 2-chloro-3-oxobutanoate
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| Synonyms |
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder-20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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|
Biological Activity
| ln Vitro |
In vitro activity:
Kinase Assay:
Cell Assay: |
|
| ln Vivo |
|
| Animal Protocol |
|
| References |
J Mol Biol.1989 Mar 20;206(2):295-304.
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Solubility Data
| Solubility (In Vitro) |
|
| Solubility (In Vivo) |
(Please use freshly prepared in vivo formulations for optimal results.)
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| Preparing Stock Solutions |
|
1 mg |
5 mg |
10 mg |
| 1 mM |
6.0757 mL |
30.3785 mL |
60.7570 mL |
| 5 mM |
1.2151 mL |
6.0757 mL |
12.1514 mL |
| 10 mM |
0.6076 mL |
3.0379 mL |
6.0757 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles. |
