9-Methoxycamptothecin (MCPT), extracted from Camptotheca acuminata, exerts anti-tumor activity by inhibiting topoisomerase. 9-Methoxycamptothecin (MCPT) has a strong effect on inducing apoptosis of G2/M phase cells and cancer/tumor cells.

Physicochemical Properties

Molecular Formula	C21H18N2O5
Molecular Weight	378.38
Exact Mass	378.121
CAS #	39026-92-1
PubChem CID	123617
Appearance	White to yellow solid powder
Density	1.5±0.1 g/cm3
Boiling Point	773.1±60.0 °C at 760 mmHg
Melting Point	223-225 ºC
Flash Point	421.4±32.9 °C
Vapour Pressure	0.0±2.8 mmHg at 25°C
Index of Refraction	1.723
LogP	2
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	2
Heavy Atom Count	28
Complexity	790
Defined Atom Stereocenter Count	1
SMILES	CC[C@@]1(C2=C(COC1=O)C(=O)N3CC4=CC5=C(C=CC=C5OC)N=C4C3=C2)O
InChi Key	XVMZDZFTCKLZTF-NRFANRHFSA-N
InChi Code	InChI=1S/C21H18N2O5/c1-3-21(26)14-8-16-18-11(7-12-15(22-18)5-4-6-17(12)27-2)9-23(16)19(24)13(14)10-28-20(21)25/h4-8,26H,3,9-10H2,1-2H3/t21-/m0/s1
Chemical Name	(S)-4-ethyl-4-hydroxy-10-methoxy-1,12-dihydro-14H-pyrano[3',4'
Synonyms	NSC 176323 NSC-176323NSC176323 9-Methoxycamptothecin
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. 9-methoxycamptothecin from Nothapodytes foetida induces apoptosis in murine sarcoma S180 cells. Z Naturforsch C. 2011 Sep-Oct;66(9-10):471-6. [2]. TNFα and Fas/FasL pathways are involved in 9-Methoxycamptothecin-induced apoptosis in cancer cells with oxidative stress and G2/M cell cycle arrest. Food Chem Toxicol. 2013 May;55:396-410.
Additional Infomation	9-Methoxycamptothecin is a pyranoindolizinoquinoline. 9-Methoxycamptothecin has been reported in Ophiorrhiza liukiuensis, Nothapodytes nimmoniana, and other organisms with data available.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~264.28 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 0.83 mg/mL (2.19 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 0.83 mg/mL (2.19 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.6428 mL	13.2142 mL	26.4285 mL
	5 mM	0.5286 mL	2.6428 mL	5.2857 mL
	10 mM	0.2643 mL	1.3214 mL	2.6428 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.