9-Fluorenylmethyl carbazate is a fluorophore reagent that can be used for fluorescence detection of sugar chains.

Physicochemical Properties

Molecular Formula	C15H14N2O2
Molecular Weight	254.2839
Exact Mass	254.105
CAS #	35661-51-9
PubChem CID	161883
Appearance	White to off-white solid powder
Density	1.3±0.1 g/cm3
Boiling Point	485.8±14.0 °C at 760 mmHg
Melting Point	-170ºC (dec.)
Flash Point	247.6±20.1 °C
Vapour Pressure	0.0±1.2 mmHg at 25°C
Index of Refraction	1.632
LogP	2.88
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	3
Heavy Atom Count	19
Complexity	312
Defined Atom Stereocenter Count	0
InChi Key	YGCGPEUVGHDMLO-UHFFFAOYSA-N
InChi Code	InChI=1S/C15H14N2O2/c16-17-15(18)19-9-14-12-7-3-1-5-10(12)11-6-2-4-8-13(11)14/h1-8,14H,9,16H2,(H,17,18)
Chemical Name	9H-fluoren-9-ylmethyl N-aminocarbamate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. A practical method for preparing fluorescent-labeled glycans with a 9-fluorenylmethyl derivative to simplify a fluorimetric HPLC-based analysis. J Pharm Biomed Anal. 2020 Jul 15;186:113267.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~196.63 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.25 mg/mL (4.92 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (4.92 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.9327 mL	19.6634 mL	39.3267 mL
	5 mM	0.7865 mL	3.9327 mL	7.8653 mL
	10 mM	0.3933 mL	1.9663 mL	3.9327 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.