7BIO (7-Bromoidirubin-3-Oxime) is an analogue of indirubin. 7BIO (7-Bromoidirubin-3-Oxime) has inhibitory effects on cyclin-dependent kinase 5 (CDK5) and glycogen synthase kinase-3β (GSK3β). 7BIO (7-Bromoidirubin-3-Oxime) inhibits Aβ oligomer-induced neuroinflammation, synaptic damage, tau hyperphosphorylation, astrocyte and microglia activation, and alleviates Aβ oligomer-induced Cognitive impairment in mice [1].

Physicochemical Properties

Molecular Formula	C16H10BRN3O2
Molecular Weight	356.17
Exact Mass	354.996
CAS #	916440-85-2
PubChem CID	135423792
Appearance	Brown to reddish brown solid powder
Density	1.80±0.1 g/cm3 (20 °C, 760 mmHg)
Boiling Point	565.5±50.0 °C (760 mmHg)
LogP	3.692
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	1
Heavy Atom Count	22
Complexity	438
Defined Atom Stereocenter Count	0
InChi Key	HYMACPDEJIEMST-UHFFFAOYSA-N
InChi Code	InChI=1S/C16H10BrN3O2/c17-10-6-3-5-9-12(16(21)19-13(9)10)15-14(20-22)8-4-1-2-7-11(8)18-15/h1-7,18-19,21H
Chemical Name	7-bromo-3-(3-nitroso-1H-indol-2-yl)-1H-indol-2-ol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	CDK5 GSK3β
ln Vitro	In SH-SY5Y cells, 7BIO (1 and 10 μM; 24 hours) exhibits neuroprotective properties and can stop Aβ1–42 oligomer-induced cell death, but not reverse it[1].
ln Vivo	In mice, the effects of Aβ oligomer-induced impairment on recognition, spatial learning, and memory are dramatically reduced by 7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection)[1]. In mice, Aβ oligomer-induced increases in TNF-α and IL-6 production in the brain and synapsin-1 and PSD-95 expression in the hippocampus are reduced by 7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection)[1]. Activation of microglia, astrogliosis, and increased expression of pTau in the mouse brain caused by Aβ oligomer are attenuated by 7Bio (2.3, 7.0, and 23.3 μg /kg; bilateral ventricle injection)[1]. Preventing decreased expression of pSer9-GSK3β and having no significant influence on the level of Tau protein is possible with 7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection)[1].
Cell Assay	Cell Viability Assay[1] Cell Types: SH-SY5Y cells Tested Concentrations: 1 and 10 μM Incubation Duration: 24 hrs (hours) Experimental Results: Inhibit Aβ oligomer-induced neuronal death. 7BIO prevented, but did not rescue Aβ1 −42 oligomer-induced cell death in SH-SY5Y cells.
Animal Protocol	Animal/Disease Models: 8 weeks mice (30 g)[1] Doses: 2.3, 7.0, and 23.3 μg/kg Route of Administration: bilateral ventricle injection Experimental Results: Attenuates Aβ oligomer-induced impairment of recognition, spatial learning and memory in mice.
References	[1]. Indirubin Derivative 7-Bromoindirubin-3-Oxime (7Bio) Attenuates Aβ Oligomer-Induced Cognitive Impairments in Mice. Front Mol Neurosci. 2017;10:393.

Solubility Data

Solubility (In Vitro)

DMSO : 250 mg/mL (701.91 mM) Ethanol : 50 mg/mL (140.38 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.02 mM) (saturation unknown) in 10% EtOH + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear EtOH stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.84 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.8076 mL	14.0382 mL	28.0765 mL
	5 mM	0.5615 mL	2.8076 mL	5.6153 mL
	10 mM	0.2808 mL	1.4038 mL	2.8076 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.