7-Methoxyflavone is a compound extracted from Zornia brasiliensis. 7-Methoxyflavone has peripheral analgesic activity. 7-Methoxyflavone can inhibit the paw licking time (65.6%) in the neurogenic phase of the formalin pain response, but does not reduce the nociceptive response in the inflammatory phase.

Physicochemical Properties

Molecular Formula	C16H12O3
Molecular Weight	252.26468
Exact Mass	252.078
CAS #	22395-22-8
PubChem CID	466268
Appearance	Off-white to brown solid powder
Density	1.2±0.1 g/cm3
Boiling Point	421.2±45.0 °C at 760 mmHg
Melting Point	110-112 °C(lit.)
Flash Point	200.3±15.1 °C
Vapour Pressure	0.0±1.0 mmHg at 25°C
Index of Refraction	1.614
LogP	3.47
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	2
Heavy Atom Count	19
Complexity	368
Defined Atom Stereocenter Count	0
InChi Key	QKNDCRMJDZLFEG-UHFFFAOYSA-N
InChi Code	InChI=1S/C16H12O3/c1-18-12-7-8-13-14(17)10-15(19-16(13)9-12)11-5-3-2-4-6-11/h2-10H,1H3
Chemical Name	7-methoxy-2-phenylchromen-4-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Antinociceptive effect of 7-methoxyflavone isolated from Zornia brasiliensis. Nat Prod Res. 2013;27(18):1695-1699.
Additional Infomation	7-Methoxyflavone is a member of flavonoids and an ether. 7-Methoxyflavone has been reported in Conchocarpus heterophyllus, Pimelea simplex, and other organisms with data available.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~396.42 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.91 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.91 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (9.91 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.9642 mL	19.8208 mL	39.6416 mL
	5 mM	0.7928 mL	3.9642 mL	7.9283 mL
	10 mM	0.3964 mL	1.9821 mL	3.9642 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.