7-Dimethylaminocoumarin-4-acetic acid is a fluorescent probe.

Physicochemical Properties

Molecular Formula	C13H13NO4
Molecular Weight	247.25
Exact Mass	247.084
CAS #	80883-54-1
PubChem CID	2762622
Appearance	Light brown to brown solid powder
Density	1.3±0.1 g/cm3
Boiling Point	497.6±45.0 °C at 760 mmHg
Flash Point	254.8±28.7 °C
Vapour Pressure	0.0±1.3 mmHg at 25°C
Index of Refraction	1.619
LogP	2.24
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	18
Complexity	389
Defined Atom Stereocenter Count	0
InChi Key	HQMBLJOHUDYJLK-UHFFFAOYSA-N
InChi Code	InChI=1S/C13H13NO4/c1-14(2)9-3-4-10-8(5-12(15)16)6-13(17)18-11(10)7-9/h3-4,6-7H,5H2,1-2H3,(H,15,16)
Chemical Name	2-[7-(dimethylamino)-2-oxochromen-4-yl]acetic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. First total synthesis of a fluorescent didemnin. Tetrahedron, 2000, 56(23): 3687-3690.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (404.45 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (10.11 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.0445 mL	20.2224 mL	40.4449 mL
	5 mM	0.8089 mL	4.0445 mL	8.0890 mL
	10 mM	0.4044 mL	2.0222 mL	4.0445 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.